Invitrogen

Cathepsin B Polyclonal Antibody

查看其他22个Cathepsin B抗体

抗体检测数据 (6)

Cathepsin B Antibody in Immunocytochemistry (ICC/IF)

Cathepsin B Antibody in Immunocytochemistry (ICC/IF)

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Cathepsin B Antibody (PA5-142155) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Cathepsin B in U2OS cells using Cathepsin B Polyclonal Antibody (Product # PA5-142155). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative ... View More

Cathepsin B Antibody in Immunocytochemistry (ICC/IF)

Cathepsin B Antibody in Immunocytochemistry (ICC/IF)

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cathepsin B Antibody in Flow Cytometry (Flow)

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Cathepsin B Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Cathepsin B in U2OS cells using Cathepsin B Polyclonal Antibody (Product # PA5-142155). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Cathepsin B Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Cathepsin B in HeLa cells using Cathepsin B Polyclonal Antibody (Product # PA5-142155). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cathepsin B in Human Small Intestine (Formalin-Fixed, Paraffin-Embedded). Samples were incubated with Cathepsin B polyclonal antibody (Product # PA5-142155).

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cathepsin B in Human Thyroid (Formalin-Fixed, Paraffin-Embedded). Samples were incubated with Cathepsin B polyclonal antibody (Product # PA5-142155).

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cathepsin B in Human Uterus (Formalin-Fixed, Paraffin-Embedded). Samples were incubated with Cathepsin B polyclonal antibody (Product # PA5-142155).

Cathepsin B Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of Cathepsin B in HepG2cells (blue line) using Cathepsin B Polyclonal Antibody (Product # PA5-142155). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody overnight (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

产品信息

PA5-142155

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-DELVNYVNKRNT.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2932813

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

靶标信息

Cathepsin B is a lysosomal cysteine protease that has been implicated in the pathogenesis of rheumatoid arthritis, muscular dystrophy and tumor metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; Cathepsin B1; cysteine protease

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基因别名: APPS; CPSB; CTSB

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UniProt ID: (Human) P07858

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Entrez Gene ID: (Human) 1508

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cysteine-type endopeptidase activity serine-type endopeptidase activity protein binding collagen binding peptidase activity cysteine-type peptidase activity kininogen binding peptide binding proteoglycan binding protein self-association cysteine protease

toll-like receptor signaling pathway proteolysis autophagy spermatogenesis skeletal muscle tissue development response to wounding response to glucose response to organic cyclic compound response to amine collagen catabolic process epithelial cell differentiation regulation of apoptotic process response to peptide hormone response to ethanol decidualization viral entry into host cell regulation of catalytic activity proteolysis involved in cellular protein catabolic process negative regulation of cell death response to interleukin-4 cellular response to mechanical stimulus cellular response to thyroid hormone stimulus