Invitrogen

Cathepsin L Polyclonal Antibody

Cathepsin L Antibody in Western Blot (WB)

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Cathepsin L Antibody (PA5-88413) in WB

Western blot analysis of Cathepsin L in extracts from LO2 cells. Samples were incubated with Cathepsin L Polyclonal antibody (Product # PA5-88413) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Cathepsin L Antibody in Western Blot, Immunocytochemistry, Proximity Ligation Assay (PLA) (WB, ICC/IF, PLA)

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产品信息

PA5-88413

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

1 µg/mL

in situ PLA (PLA)

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产品规格

种属反应

Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 114-333 of human Cathepsin L/V/K/H (NP_0019031)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.99 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804895

产品详细信息

Immunogen sequence: APRSVDWREK GYVTPVKNQG QCGSCWAFSA TGALEGQMFR KTGRLISLSE QNLVDCSGPQ GNEGCNGGLM DYAFQYVQDN GGLDSEESYP YEATEESCKY NPKYSVANDT GFVDIPKQEK ALMKAVATVG PISVAIDAGH ESFLFYKEGI YFEPDCSSED MDHGVLVVGY GFESTESDNN KYWLVKNSWG EEWGMGGYVK MAKDRRNHCG IASAASYPTV; Positive Samples: LO2, 293T, Rat kidney; Cellular Location: Lysosome

靶标信息

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress.

Cell death & disease

Chaumonnot K,Masson S,Sikner H,Bouchard A,Baverel V,Bellaye PS,Collin B,Garrido C,Kohli E

PA5-88413 was used in Immunocytochemistry, Proximity Ligation Assay (PLA), Western Blot to investigate intracellular and cell surface expression the endoplasmic reticulum stress protein GRP94 in M1 and M2 macrophages derived from PBMC of healthy volunteers.