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Cdc20 Antibody (PA5-90600) in IHC (P)
Immunohistochemistry analysis of Cdc20 in paraffin-embedded human thyroid cancer. Samples were incubated with Cdc20 Polyclonal antibody (Product # PA5-90600) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-90600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CDC20 (NP_0012462?)
Conjugate
Form
Liquid
Concentration
1.172 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806139
Product Specific Information
Positive Samples: LO2, HT-29, A-431; Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Immunogen sequence: MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ
Target Information
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC20 cell division cycle 20 homolog; cell cycle protein p55CDC; cell division cycle 20 homolog; Cell division cycle protein 20 homolog; mmCdc20; p55CDC
Gene Aliases: 2310042N09Rik; bA276H19.3; C87100; CDC20; CDC20A; p55CDC
UniProt ID: (Human) Q12834, (Rat) Q62623, (Mouse) Q9JJ66
Entrez Gene ID: (Human) 991, (Rat) 64515, (Mouse) 107995
cell cycle
sister chromatid cohesion
mitotic nuclear division
positive regulation of cell proliferation
anaphase-promoting complex-dependent catabolic process
positive regulation of synaptic plasticity
regulation of meiotic nuclear division
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of dendrite development
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of synapse maturation
positive regulation of ubiquitin protein ligase activity
protein ubiquitination
nervous system development
cell differentiation
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