Invitrogen

Cdc42 Polyclonal Antibody

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抗体检测数据 (4)
高级验证 (1)

Cdc42 Antibody in Immunocytochemistry (ICC/IF)

Cdc42 Antibody in Immunocytochemistry (ICC/IF)

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Cdc42 Antibody (PA5-20764) in ICC/IF

Immunofluorescence analysis of cdc42 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cdc42 Polyclonal Antibody (Product # PA5-20764) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 (Product # A-11039) at a dilution of 1:2000 for... View More

Cdc42 Antibody in Immunocytochemistry (ICC/IF)

Cdc42 Antibody in Western Blot (WB)

Cdc42 Antibody in Western Blot (WB)

Cdc42 Antibody in Western Blot (WB)

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Cdc42 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of cdc42 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cdc42 Polyclonal Antibody (Product # PA5-20764) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 (Product # A-11039) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal and cytoplasmic localization. Panel e represents cells with no primary antibody to assess background. The images were captured at 60X magnification.

Cdc42 Antibody in Western Blot (WB) — Western blot was performed using Anti-Cdc42 Polyclonal Antibody (Product # PA5-20764) and 21 kDa band corresponding to Cdc42 was observed across cell lines and tissues tested along with certain uncharacterized bands at ~ 80 kDa. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), U-937 (Lane 2), A-431 (Lane 3), K-562 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), tissue extracts of Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5µg/mL) and detected by chemiluminescence Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Cdc42 Antibody in Western Blot (WB) — KD of Cdc42 was achieved by transfecting A-431 with Cdc42 specific siRNAs (Silencer® select Product # s2765, s2766). Western blot analysis (Fig. a) was performed using whole cell extracts from the Cdc42 KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Cdc42 Polyclonal Antibody (Product # PA5-20764, 0.5µg/mL) and Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cdc42.

Cdc42 Antibody in Western Blot (WB) — Western Blot analysis of CDC42 in human brain tissue lysate with Cdc42 Polyclonal Antibody (Product # PA5-20764) at (A) 0.5 and (B) 1 µg/mL.

Cdc42 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. A-431 cells were transfected with Cdc42 siRNA and reduction of signal was observed in Western Blot using Cdc42 Polyclonal Antibody (Product # PA5-20764). {KD}

产品信息

PA5-20764

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

-

免疫细胞化学 (ICC/IF)

5 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

A 17 amino acid peptide near the amino terminus of human CDC42.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11153253

产品详细信息

A suggested positive control is human brain tissue lysate.

PA5-20764 can be used with blocking peptide PEP-0878.

靶标信息

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein; RP1-224A6.5; small GTP binding protein CDC42

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基因别名: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS

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UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P25763

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Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540

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GTPase activity protein binding GTP binding protein kinase binding GTP-dependent protein binding mitogen-activated protein kinase kinase kinase binding thioesterase binding apolipoprotein A-I receptor binding identical protein binding Rho GDP-dissociation inhibitor binding ubiquitin protein ligase activity nucleotide binding

positive regulation of protein phosphorylation sprouting angiogenesis cardiac conduction system development keratinocyte development endocytosis actin filament organization Golgi organization regulation of mitotic nuclear division nuclear migration small GTPase mediated signal transduction metabolic process response to glucose positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion regulation of exocytosis substantia nigra development establishment of cell polarity actin cytoskeleton organization hair follicle morphogenesis positive regulation of pseudopodium assembly negative regulation of protein complex assembly keratinization regulation of protein stability positive regulation of cytokinesis positive regulation of peptidyl-serine phosphorylation adherens junction organization cellular protein localization establishment or maintenance of apical/basal cell polarity multicellular organism growth dendritic cell migration regulation of protein catabolic process positive regulation of catalytic activity positive regulation of MAPK cascade regulation of protein heterodimerization activity positive regulation of neuron apoptotic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication regulation of protein kinase activity positive regulation of JNK cascade filopodium assembly neuron fate determination epidermis morphogenesis actin filament bundle assembly regulation of protein metabolic process regulation of filopodium assembly nucleus localization establishment of Golgi localization positive regulation of synapse structural plasticity regulation of attachment of spindle microtubules to kinetochore heart contraction canonical Wnt signaling pathway submandibular salivary gland formation epithelial-mesenchymal cell signaling hair follicle placode formation positive regulation of hair follicle cell proliferation organelle transport along microtubule actin filament branching epithelial cell-cell adhesion positive regulation of intracellular protein transport positive regulation of substrate adhesion-dependent cell spreading establishment or maintenance of cell polarity blood coagulation protein ubiquitination macrophage differentiation positive regulation of cell growth T cell costimulation Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of epidermal growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of small GTPase mediated signal transduction positive regulation of muscle cell differentiation Wnt signaling pathway, planar cell polarity pathway positive regulation of epithelial cell proliferation involved in lung morphogenesis Rho protein signal transduction nervous system development endosomal transport cell differentiation