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Cdc6 Antibody (PA5-95150) in WB
Western Blot was performed using Anti-Cdc6 Polyclonal Antibody (Product # PA5-95150) and a 62 kDa band corresponding to CDC6 was observed in tissue extracts and showed differential expression upon treatments in cell lines. Whole cell extracts (40 µg lysate) from asynchronous HeLa (Lane 1), serum starved HeLa (0.1% FBS for 72 hr) (Lane 2), serum starved (0.1% FBS for 72 hr) and serum released HeLa (10% FBS for 20 hr) (Lane 3), asynchronous T98G (Lane 4), serum starved and released T98G (Lane 5), serum starved T98G (Lane 6), untreated HCT-11... View More
产品信息
PA5-95150
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc6 (484-503aa KLYEAYSKVCRKQQVAAVDQ).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2806955
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; Cell division control protein 6 homolog; cell division cycle 6 homolog; HsCdc18; HsCDC6; p62(cdc6)
基因别名: CDC18L; CDC6; HsCDC18; HsCDC6
UniProt ID: (Human) Q99741
Entrez Gene ID: (Human) 990
DNA replication checkpoint
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
DNA replication
DNA replication initiation
mitotic nuclear division
traversing start control point of mitotic cell cycle
negative regulation of DNA replication
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
positive regulation of cytokinesis
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of fibroblast proliferation
cell division
positive regulation of chromosome segregation
cellular response to vasopressin
cellular response to angiotensin
