Invitrogen

ChAT Polyclonal Antibody

ChAT Antibody in Immunocytochemistry (ICC/IF)

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ChAT Antibody (PA5-77838) in ICC/IF

Immunofluorescent analysis of ChAT in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ChAT polyclonal antibody (Product # PA5-77838) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) Choline Acetyltransferase Antibody D) Merged image... View More

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产品信息

PA5-77838

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2735637

产品详细信息

A 1:1,000 dilution of PA5-77838 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Predicted molecular weight at approximately 82.5kDa. Observed molecular weights between 68-70kDa.