Invitrogen

ChAT Monoclonal Antibody (CL3173)

ChAT Antibody in Immunocytochemistry (ICC/IF)

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ChAT Antibody (MA5-31383) in ICC/IF

Immunofluorecent analysis of ChAT in SK-MEL-30 cells using ChAT Monoclonal Antibody (CL3173) (Product # MA5-31383). Staining shows specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

ChAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ChAT Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ChAT Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across brain and kidney lysates of Mouse and Rat tissues owing to their inherent genetic constitution. Relative expression of acetyl CoA: choline O-acetyltransferase was observed higher in Mouse and Rat brain tissue lysates in comparison to Mouse and Rat Kidney lysates using Anti-ChAT Monoclonal Antibody (CL3173) (Product # MA5-31383) in Western Blot. {RE}

产品信息

MA5-31383

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

2-10 µg/ml

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

CL3173

抗原

Recombinant protein corresponding to Human CHAT

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2787020

产品详细信息

Immunogen sequence: GLFSSYRLPG HTQDTLVAQN SSIMPEPEHV IVACCNQFFV LDVVINFRRL SEGDLFTQLR KIVKMASNED ERLPPIGLLT SDGRSEWAEA RTVLVKDSTN

靶标信息

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases.

Scientific reports

Yamamotoya T,Hasei S,Akasaka Y,Ohata Y,Nakatsu Y,Kanna M,Fujishiro M,Sakoda H,Ono H,Kushiyama A,Misawa H,Asano T

MA5-31383 was used in Immunohistochemistry to clarify that loss of motor neuron TFG is sufficient for the occurrence of NMJ degeneration and muscle atrophy, though lack of muscle TFG may exert an additional effect.