Invitrogen

Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 647

Clathrin Heavy Chain Antibody in Immunocytochemistry (ICC/IF)

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Clathrin Heavy Chain Antibody (MA1-065-A647) in ICC/IF

Immunofluorescent analysis of Clathrin Heavy Chain (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA1-065-A647) at a dilution of 5 µg/mL in blocking buffer fo... View More

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产品信息

MA1-065-A647

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

X22

抗原

Purified human brain clathrin heavy chain

查看免疫原

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2662917

产品详细信息

MA1065A647 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.

The MA1065A647 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A647 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

靶标信息

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images.

The Journal of cell biology

Vega-Lugo J,da Rocha-Azevedo B,Dasgupta A,Jaqaman K

MA1-065-A647 was used in Immunocytochemistry-immunofluorescence to describe an approach, termed "conditional colocalization analysis," for analyzing the colocalization relationships between three molecular entities in three-color microscopy images.

Mon Jul 04 00:00:00 UTC 2022

生物信息学

蛋白别名: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22

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基因别名: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732