Invitrogen

Claudin 12 Polyclonal Antibody

Claudin 12 Antibody in Immunocytochemistry (ICC/IF)

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Claudin 12 Antibody (38-8200) in ICC/IF

Immunofluorescent analysis of Claudin-12 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Claudin-12 rabbit polyclonal Antibody (Product # 38-8200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room tempe... View More

Claudin 12 Antibody in Western Blot (WB)

Claudin 12 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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产品信息

38-8200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the N-terminal region of mouse and human Claudin-12

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533384

靶标信息

The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Organic osmolytes increase expression of specific tight junction proteins in skin and alter barrier function in keratinocytes.

The British journal of dermatology

El-Chami C,Foster AR,Johnson C,Clausen RP,Cornwell P,Haslam IS,Steward MC,Watson REB,Young HS,O'Neill CA

38-8200 was used in Immunohistochemistry to investigate whether osmolytes can modify tight junctions.

Mon Mar 01 00:00:00 UTC 2021

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.

International journal of molecular sciences

Markov AG,Fedorova AA,Kravtsova VV,Bikmurzina AE,Okorokova LS,Matchkov VV,Cornelius V,Amasheh S,Krivoi II

38-8200 was used in Western Blotting to confirm that circulating ouabain can functionally and tissue-specifically affect barrier properties of epithelial and endothelial tissues via Na,K-ATPase-mediated modulation of claudins expression.

Fri Jul 17 00:00:00 UTC 2020

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.

International journal of molecular sciences

Markov AG,Fedorova AA,Kravtsova VV,Bikmurzina AE,Okorokova LS,Matchkov VV,Cornelius V,Amasheh S,Krivoi II

Fri Jul 17 00:00:00 UTC 2020

Glycocholic acid and butyrate synergistically increase vitamin D-induced calcium uptake in Caco-2 intestinal epithelial cell monolayers.

Bone reports

Casselbrant A,Fändriks L,Wallenius V

38-8200 was used in Western Blotting to identify mechanisms in vitro that may be responsible for the decreased calcium absorption after Roux-en-Y gastric bypass.

Tue Dec 01 00:00:00 UTC 2020

Claudin-12 is not required for blood-brain barrier tight junction function.

Fluids and barriers of the CNS

Castro Dias M,Coisne C,Baden P,Enzmann G,Garrett L,Becker L,Hölter SM,Hrabě de Angelis M,Deutsch U,Engelhardt B

38-8200 was used in Western Blotting to study the role and localisation of claudin-12.

Thu Sep 12 00:00:00 UTC 2019

Claudin-12 is not required for blood-brain barrier tight junction function.

Fluids and barriers of the CNS

Castro Dias M,Coisne C,Baden P,Enzmann G,Garrett L,Becker L,Hölter SM,Hrabě de Angelis M,Deutsch U,Engelhardt B

38-8200 was used in Western Blotting to study the role and localisation of claudin-12.

Thu Sep 12 00:00:00 UTC 2019

Claudin-12 is not required for blood-brain barrier tight junction function.

Fluids and barriers of the CNS

Castro Dias M,Coisne C,Baden P,Enzmann G,Garrett L,Becker L,Hölter SM,Hrabě de Angelis M,Deutsch U,Engelhardt B

38-8200 was used in Western Blotting to study the role and localisation of claudin-12.

Thu Sep 12 00:00:00 UTC 2019

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Tissue barriers

Chen H,Lu R,Zhang YG,Sun J

38-8200 was used in Western Blotting to study the role of the vitamin D receptor in lung barrier integrity.

Mon Apr 01 00:00:00 UTC 2019

Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression.

Nutrition research (New York, N.Y.)

Kühne H,Hause G,Grundmann SM,Schutkowski A,Brandsch C,Stangl GI

38-8200 was used in western blot to characterize the exhibition of elongated intestinal microvilli and increased ezrin expression due to vitamin D receptor knockout mice

Mon Feb 01 00:00:00 UTC 2016

Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet.

PloS one

Elfers K,Marr I,Wilkens MR,Breves G,Langeheine M,Brehm R,Muscher-Banse AS

38-8200 was used in western blot to characterize a reduced N and/or Ca diet by expression of tight junction proteins and cadherin 17 in the small intestine of young goats

Mon Mar 27 00:00:00 UTC 2017

Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node.

European journal of immunology

Pfeiffer F,Kumar V,Butz S,Vestweber D,Imhof BA,Stein JV,Engelhardt B

38-8200 was used in immunohistochemistry to examine the architecture of cellular junctions present in blood and lymphatic vessel endothelium in peripheral lymph nodes