Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 4
Claudin 16 Antibody (34-5400) in ICC/IF
Immunofluorescence analysis of Claudin 16 was performed using 90% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin 16 Rabbit Polyclonal Antibody (Product # 34-5400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at ro... View More
产品信息
34-5400
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Dog,
Human,
Mouse,
Plant,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the C-terminal region of human Claudin 16.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533174
靶标信息
Tight junction group of proteins are a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junction strands are mainly composed of claudins, occludin, and JAM. Claudin-16 belongs to the Claudin family of protein which is composed of 23 integral membrane proteins. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1. Claudin 16, also known as Paracellin-1, is a renal tight junction protein required for paracellular magnesium resorption. It is localized in the intercellular junctions of the epithelial cells of the thick ascending limb of Henle's loop (a TAL marker) are used to localize this specific structure of nephrons in kidney sections. Mg2+ resorption occurs in TAL, and Claudin-16 is required for this paracellular function. Failure in renal 'handling' of magnesium can result in hypermagnesemia, whose major direct toxicity implications are cardiovascular as well as renal failure. Recent studies have shown that the lack of Claudin-16 proteins contributes to the dysfunction of paracellular renal transport systems.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Claudin-16; H59D2a protein; hypomagnesemia 3, with hypercalciuria and nephrocalcinosis; Paracellin-1; PCLN-1
基因别名: claudin-16; CLDN16; HOMG3; PCLN1
UniProt ID: (Human) Q9Y5I7, (Mouse) Q925N4
Entrez Gene ID: (Human) 10686, (Mouse) 114141
