Invitrogen

Claudin 5 Polyclonal Antibody

Claudin 5 Antibody in Immunocytochemistry (ICC/IF)

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Claudin 5 Antibody (PA5-99415) in ICC/IF

Immunofluorescent analysis of Claudin 5 in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Claudin 5 polyclonal antibody (Product # PA5-99415) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Claudin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Claudin 5 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

PA5-99415

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:100-1:500

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产品规格

种属反应

Bovine, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CLDN5(Accession O00501), corresponding to amino acid residues L181-V218.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2818348

产品详细信息

Antibody detects endogenous levels of total Claudin 5.

靶标信息

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury.

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PA5-99415 was used in Immunohistochemistry to indicate that FTY-720 has promise as a new therapy for neurovascular protection and functional recovery after ischemic stroke.

Sat Apr 01 00:00:00 UTC 2023

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Neural regeneration research

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PA5-99415 was used in Immunohistochemistry to indicate that FTY-720 has promise as a new therapy for neurovascular protection and functional recovery after ischemic stroke.

Sat Apr 01 00:00:00 UTC 2023

Blood-Brain Barrier Rescue by Roflumilast After Transient Global Cerebral Ischemia in Rats.

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PA5-99415 was used in Immunohistochemistry to investigate whether roflumilast confers BBB neuroprotection in the hippocampus after transient global cerebral ischemia (TGCI) in rats.

Tue Aug 01 00:00:00 UTC 2023

Cholesterol crystal formation is a unifying pathogenic mechanism in the development of diabetic retinopathy.

Diabetologia

Hammer SS,Dorweiler TF,McFarland D,Adu-Agyeiwaah Y,Mast N,El-Darzi N,Fortmann SD,Nooti S,Agrawal DK,Pikuleva IA,Abela GS,Grant MB,Busik JV

PA5-99415 was used in Immunocytochemistry to establish that cholesterol accumulation and CC formation are a unifying pathogenic mechanism in the development of diabetic retinopathy.

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Inhibiting PI3K/Akt-Signaling Pathway Improves Neurobehavior Changes in Anti-NMDAR Encephalitis Mice by Ameliorating Blood-Brain Barrier Disruption and Neuronal Damage.

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PA5-99415 was used in Immunohistochemistry and Western Blotting to show that the activation of PI3K/Akt, along with the changes in TJ-related proteins ZO-1 and Claudin-5, may be closely related to BBB damage and neurobehavior changes in anti-NMDAR encephalitis mice.

Sun Oct 01 00:00:00 UTC 2023

Inhibiting PI3K/Akt-Signaling Pathway Improves Neurobehavior Changes in Anti-NMDAR Encephalitis Mice by Ameliorating Blood-Brain Barrier Disruption and Neuronal Damage.

Cellular and molecular neurobiology

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生物信息学

蛋白别名: Brain endothelial cell clone 1 protein; claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin-5; Lung-specific membrane protein; TMDVCF; Transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome

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基因别名: AI854493; AWAL; BEC1; CLDN5; CPETRL1; MBEC1; TMVCF