Invitrogen

Collagen I Polyclonal Antibody

Collagen I Antibody in Immunocytochemistry (ICC/IF)

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Collagen I Antibody (PA5-95137) in ICC/IF

Immunofluorescence analysis of Collagen I in NIH3T3 cell. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen I polyclonal antibody (Product # PA5-95137) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) using a dilution and developed with Strepavidin-Biotin-Complex/DAB.

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Collagen I Antibody in Western Blot (WB)

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产品信息

PA5-95137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2806942

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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PA5-95137 was used in Immunohistochemistry to demonstrate the utility of in vivo proximity labeling for dissecting local control of complex systems and reveals new mechanisms for dictating RGC integrity and cortical architecture.

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FBXL6 is dysregulated in keloids and promotes keloid fibroblast growth by inducing c-Myc expression.

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Feng G,Sun H,Piao M

PA5-95137 was used in Western Blotting to investigate the role of FBXL6 in keloid fibroblasts, particularly its impact on cell proliferation and collagen deposition, and to explore its potential as a therapeutic target for keloid treatment.

Sun Jan 01 00:00:00 UTC 2023

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

PA5-95137 was used in Western Blotting and Immunohistochemistry to show that pharmacological fibroblast activation protein (FAP) inhibition attenuates inflammation-predominant liver fibrosis. FAP is expressed on subsets of activated fibroblasts/hepatic stellate cells (HSC) and promotes both macrophage and HSC profibrogenic activity in liver fibrosis.

Tue Mar 21 00:00:00 UTC 2023

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

Tue Mar 21 00:00:00 UTC 2023

Myelofibrosis progression grading based on type I and type III collagen and fibrillin 1 expression boosted by whole slide image analysis.

Histopathology

Szekely T,Wichmann B,Maros ME,Csizmadia A,Bodor C,Timar B,Krenacs T

PA5-95137 was used in Immunohistochemistry to study the expression of the major extracellular matrix proteins of fibrosis in relation to diagnostic silver grading.

Wed Mar 01 00:00:00 UTC 2023

Muscle stem cells and fibro-adipogenic progenitors in female pelvic floor muscle regeneration following birth injury.

NPJ Regenerative medicine

Sesillo FB,Rajesh V,Wong M,Duran P,Rudell JB,Rundio CP,Baynes BB,Laurent LC,Sacco A,Christman KL,Alperin M

PA5-95137 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate that the loss of function and decreased expansion of MuSCs and FAPs after birth injury lead to impaired PFM recovery.

Fri Dec 16 00:00:00 UTC 2022

Development of skeletal muscle atrophy and intermuscular adipose tissue in patients with early breast cancer treated with chemotherapy.

American journal of physiology. Cell physiology

Mallard J,Hucteau E,Bender L,Charlot A,Debrut L,Pflumio C,Trensz P,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

PA5-95137 was used in Western Blotting to demonstrate both IMAT development and muscle atrophy in patients with breast cancer who were treated with chemotherapy.

Sat Oct 01 00:00:00 UTC 2022

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

PA5-95137 was used in Western Blot to suggest that paclitaxel modifies gene and protein expression of skin-related molecular markers, and impairs different physical, physiological, and biomechanical properties of the skin of cancer patients at a subclinical level.

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Additively manufactured BaTiO₃ composite scaffolds: A novel strategy for load bearing bone tissue engineering applications.

Materials science & engineering. C, Materials for biological applications

Mancuso E,Shah L,Jindal S,Serenelli C,Tsikriteas ZM,Khanbareh H,Tirella A

PA5-95137 was used in Immunocytochemistry to conclude that the single-step multi-material additive manufacturing technology used for the fabrication of electroactive PCL/BaTiO3 composite scaffolds holds great promise for sustainability (reduced material waste and manufacturing costs) and it importantly suggests PCL/BaTiO3 scaffolds as promising candidates for load bearing bone tissue engineering applications to solve unmet clinical needs.

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mRNA and long non-coding RNA expression profiling of human periodontal ligament cells under tension loading.

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Published figure using Collagen I polyclonal antibody (Product # PA5-95137) in Western Blot

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LTBP4 affects renal fibrosis by influencing angiogenesis and altering mitochondrial structure.

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Published figure using Collagen I polyclonal antibody (Product # PA5-95137) in Western Blot

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Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.

Frontiers in pharmacology

El-Sherbiny M,Atef H,Eladl MA,Mohamed AS,El-Shafey M,Ali HS,Zaitone SA,Alomar SY,Alqahtani SAM,Aloyouni SY,Attia MA

PA5-95137 was used in Western Blot to examine the dose-dependent lung injury produced by leflunomide in healthy mice.

Tue May 18 00:00:00 UTC 2021

Fibrin polymer on the surface of biomaterial implants drives the foreign body reaction.

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生物信息学

蛋白别名: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

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基因别名: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim