Invitrogen

Collagen I Monoclonal Antibody (NFI/20)

产品信息

MA1-83073

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

1:20

酶联免疫吸附实验 (ELISA)

1:1,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

NFI/20

抗原

Human Collagen type I.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

Ion-exchange chromatography, Ammonium sulfate precipitation

保存液

PBS/0.01M sodium borate with 1% mannitol, 1% dextran

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_930061

产品详细信息

MA1-83073 detects Collagen I in human sample types, and does not cross-react with collagen types II, III, or IV.

Reconstitute with 1 mL of distilled water. Prior to reconstitution, store at 4ºC. After reconstitution, store undiluted at -20ºC, avoiding freeze/thaw cycles.

Mouse anti Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite.

靶标信息

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain

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基因别名: COL1A1; COL1A2; EDSC; OI1; OI2; OI3; OI4

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UniProt ID: (Human) P02452, (Human) P08123

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Entrez Gene ID: (Human) 1277, (Human) 1278

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功能

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding

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