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Collagen Type IV Antibody (600-101-MN4) in ICC/IF
Immunofluorescence of Goat Anti-Collagen Type IV Antibody. Cells: NIH/3T3 cells. Fixative: 4%PFA. Permeabilization: None. Primary Antibody: Anti-Collagen Type IV at 15µg/mL overnight at 2-8°C. Counterstain: Donkey Anti-Goat IgG Dylight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Collagen Tye IV + Dylight™488. (C) Merge A+B. (D) secondary only... View More
Product Details
600-101-MN4
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV.
Conjugate
Form
Liquid
Concentration
0.92 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Collagen IV. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.
Target Information
Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha1(IV) collagen; canstatin; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen alpha-5(IV) chain; collagen alpha-6(IV) chain; collagen type IV alpha3 chain; collagen type IV alpha5 chain; Del(8)Bru44H; procollagen type IV alpha 5; procollagen type IV alpha 6; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; procollagen, type IV, alpha 5; procollagen, type IV, alpha 6; retinal anterior wiring; tumstatin; Type IV Collagen
Gene Aliases: [a]3(IV); [a]4(IV); alpha3(IV); BB116301; Bru; Col4a-1; Col4a-2; Col4a1; Col4a2; Col4a3; Col4a4; Del(8)44H; E130010M05Rik; Raw; Svc
UniProt ID: (Mouse) P02463, (Mouse) P08122, (Mouse) Q9QZS0, (Mouse) Q9QZR9
Entrez Gene ID: (Mouse) 12826, (Mouse) 12827, (Mouse) 12828, (Mouse) 12829, (Mouse) 12830, (Mouse) 94216
angiogenesis
patterning of blood vessels
brain development
neuromuscular junction development
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
cellular response to amino acid stimulus
basement membrane organization
transcription, DNA-templated
negative regulation of angiogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
cell surface receptor signaling pathway
cell proliferation
glomerular basement membrane development
endothelial cell apoptotic process
extracellular matrix organization
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