Invitrogen

Complement C3 Polyclonal Antibody

Complement C3 Antibody in Immunocytochemistry (ICC/IF)

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Complement C3 Antibody (PA5-21349) in ICC/IF

Immunofluorescence analysis of Complement C3 was performed using 70% confluent log phase Hep G2 cells treated with 10 ng/mL of LPS for 18 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Complement C3 Polyclonal Antibody (Product # PA5-21349) at 5 ... View More

Complement C3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Complement C3 Antibody in Western Blot (WB)

Complement C3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Complement C3 Antibody in Immunohistochemistry (IHC)

Complement C3 Antibody in Flow Cytometry (Flow)

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产品信息

PA5-21349

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the C-terminal region of human C3 (aa 1448-1655)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.07 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153785

产品详细信息

Recommended positive controls: Huh7, HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Temporal dynamics of microglia-astrocyte interaction in neuroprotective glial scar formation after intracerebral hemorrhage.

Journal of pharmaceutical analysis

Zheng J,Wu H,Wang X,Zhang G,Lu J,Xu W,Xu S,Fang Y,Zhang A,Shao A,Chen S,Zhao Z,Zhang J,Yu J

PA5-21349 was used in Immunohistochemistry to investigate whether microglia-astrocyte interaction affects glial scar formation and explore the specific function of glial scar.

Tue Aug 01 00:00:00 UTC 2023

Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway.

Brain sciences

Li X,Zhou R,Peng H,Peng J,Li Q,Mei M

PA5-21349 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that microglia PKM2 inhibition ameliorates neuroinflammation and neuron loss through C3-C3aR interaction in epilepsy, which provides an attractive target for the intervention of damaged neuron-glia crosstalk in epilepsy.

Fri Feb 03 00:00:00 UTC 2023

Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels.

Nature communications

Novakovic MM,Korshunov KS,Grant RA,Martin ME,Valencia HA,Budinger GRS,Radulovic J,Prakriya M

PA5-21349 was used in Immunohistochemistry to identify Orai1 as an important signaling hub controlling astrocyte reactivity and astrocyte-mediated brain inflammation that is commonly observed in many neurological disorders.

Thu Sep 07 00:00:00 UTC 2023

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.

Journal of neuroinflammation

Wang C,Li L

PA5-21349 was used in Immunohistochemistry-immunofluorescence to demonstrate that astrocytic KLF4 expression and complement C3-positive A1 and S100 calcium binding protein A10 (S100A10)-positive A2 astrocytes were induced in the ischemic penumbra following focal cerebral ischemia, and the time course of upregulation of astrocytic KLF4 correlated closely with the activation of A2 astrocytes.

Thu Feb 23 00:00:00 UTC 2023

Activated endothelial cells induce a distinct type of astrocytic reactivity.

Communications biology

Taylor X,Cisternas P,Jury N,Martinez P,Huang X,You Y,Redding-Ochoa J,Vidal R,Zhang J,Troncoso J,Lasagna-Reeves CA

Published figure using Complement C3 polyclonal antibody (Product # PA5-21349) in Immunocytochemistry

Tue Mar 29 00:00:00 UTC 2022

Activated endothelial cells induce a distinct type of astrocytic reactivity.

Communications biology

Taylor X,Cisternas P,Jury N,Martinez P,Huang X,You Y,Redding-Ochoa J,Vidal R,Zhang J,Troncoso J,Lasagna-Reeves CA

Published figure using Complement C3 polyclonal antibody (Product # PA5-21349) in Immunocytochemistry

Tue Mar 29 00:00:00 UTC 2022

Ceria nanoparticles ameliorate white matter injury after intracerebral hemorrhage: microglia-astrocyte involvement in remyelination.

Journal of neuroinflammation

Zheng J,Lu J,Mei S,Wu H,Sun Z,Fang Y,Xu S,Wang X,Shi L,Xu W,Chen S,Yu J,Liang F,Zhang J

PA5-21349 was used in Immunohistochemistry to reveal a new mechanism in white matter injury after ICH: ICH induces M1 microglia and A1 astrocyte through ROS-induced NF-κB p65 translocation that hinders OPC maturation.

Mon Feb 15 00:00:00 UTC 2021

Ceria nanoparticles ameliorate white matter injury after intracerebral hemorrhage: microglia-astrocyte involvement in remyelination.

Journal of neuroinflammation

Zheng J,Lu J,Mei S,Wu H,Sun Z,Fang Y,Xu S,Wang X,Shi L,Xu W,Chen S,Yu J,Liang F,Zhang J

Mon Feb 15 00:00:00 UTC 2021

The Retropepsin-Type Protease APRc as a Novel Ig-Binding Protein and Moonlighting Immune Evasion Factor of Rickettsia.

mBio

Curto P,Barro A,Almeida C,Vieira-Pires RS,Simões I

Published figure using Complement C3 polyclonal antibody (Product # PA5-21349) in Flow Cytometry

Tue Dec 21 00:00:00 UTC 2021

CFH Loss in Human RPE Cells Leads to Inflammation and Complement System Dysregulation via the NF-κB Pathway.

International journal of molecular sciences

Armento A,Schmidt TL,Sonntag I,Merle DA,Jarboui MA,Kilger E,Clark SJ,Ueffing M

PA5-21349 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that in RPE cells, FH and the NF-κB pathway work in synergy to maintain inflammatory and complement balance, and in case either one of them is dysregulated, the RPE microenvironment changes towards a proinflammatory AMD-like phenotype.

Fri Aug 13 00:00:00 UTC 2021

IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation.

Arteriosclerosis, thrombosis, and vascular biology

Zhang X,Li J,Luo S,Wang M,Huang Q,Deng Z,de Febbo C,Daoui A,Liew PX,Sukhova GK,Metso J,Jauhiainen M,Shi GP,Guo J

PA5-21349 was used in Western Blotting to demonstrate that IgE deficiency reduced atherosclerosis lesion burden, lesion lipid deposition, smooth muscle cell and endothelial cell contents, chemokine MCP (monocyte chemoattractant protein)-1 expression and macrophage accumulation.

Sun Mar 01 00:00:00 UTC 2020

A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy.

Journal of neuroinflammation

Taylor X,Cisternas P,You Y,You Y,Xiang S,Marambio Y,Zhang J,Vidal R,Lasagna-Reeves CA

PA5-21349 was used in Immunocytochemistry-immunoflourescence to demonstrate that initial glial response associated with early-stage CAA is characterized by the upregulation of A1 astrocytes without significant microglial reactivity.

Sat Jul 25 00:00:00 UTC 2020

A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy.

Journal of neuroinflammation

Taylor X,Cisternas P,You Y,You Y,Xiang S,Marambio Y,Zhang J,Vidal R,Lasagna-Reeves CA

Sat Jul 25 00:00:00 UTC 2020

Complement in Reproductive White Adipose Tissue Characterizes the Obese Preeclamptic-Like BPH/5 Mouse Prior to and During Pregnancy.

Biology

Olson KN,Reijnders D,Gomes VCL,Hebert RC,Liu CC,Stephens JM,Redman LM,Douglas NC,Sones JL

PA5-21349 was used in Western Blotting to explore potential contribution of inflammatory mediators in visceral white adipose tissue (WAT) to Preeclampsia.

Tue Sep 22 00:00:00 UTC 2020

Complement in Reproductive White Adipose Tissue Characterizes the Obese Preeclamptic-Like BPH/5 Mouse Prior to and During Pregnancy.

Biology

Olson KN,Reijnders D,Gomes VCL,Hebert RC,Liu CC,Stephens JM,Redman LM,Douglas NC,Sones JL

PA5-21349 was used in Western Blotting to explore potential contribution of inflammatory mediators in visceral white adipose tissue (WAT) to Preeclampsia.

Tue Sep 22 00:00:00 UTC 2020

Loss of Complement Factor H impairs antioxidant capacity and energy metabolism of human RPE cells.

Scientific reports

Armento A,Honisch S,Panagiotakopoulou V,Sonntag I,Jacob A,Bolz S,Kilger E,Deleidi M,Clark S,Ueffing M

PA5-21349 was used in Western Blotting to suggest that endogenously produced FH contributes to transcriptional and metabolic homeostasis and protects RPE cells from oxidative stress, highlighting a novel role of FH in AMD pathogenesis.

Thu Jun 25 00:00:00 UTC 2020

Loss of Complement Factor H impairs antioxidant capacity and energy metabolism of human RPE cells.

Scientific reports

Armento A,Honisch S,Panagiotakopoulou V,Sonntag I,Jacob A,Bolz S,Kilger E,Deleidi M,Clark S,Ueffing M

Thu Jun 25 00:00:00 UTC 2020

Bacillus anthracis Poly-γ-D-Glutamate Capsule Inhibits Opsonic Phagocytosis by Impeding Complement Activation.

Frontiers in immunology

Sharma S,Bhatnagar R,Gaur D

PA5-21349 was used in Western Blotting to demonstrate the mechanism through which the B. anthracis capsule imparts a survival advantage to the active bacteria.

Thu Mar 04 00:00:00 UTC 2021

Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice.

Theranostics

Xian P,Hei Y,Wang R,Wang T,Yang J,Li J,Di Z,Liu Z,Baskys A,Liu W,Wu S,Long Q

PA5-21349 was used in Immunohistochemistry to show that MSC-Exo ameliorate inflammation-induced astrocyte alterations and that the Nrf2-NF-κB signaling pathway is involved in regulating astrocyte activation in mice.

Mon Aug 31 00:00:00 UTC 2020

Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice.

Theranostics

Xian P,Hei Y,Wang R,Wang T,Yang J,Li J,Di Z,Liu Z,Baskys A,Liu W,Wu S,Long Q

Mon Aug 31 00:00:00 UTC 2020

Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice.

Theranostics

Xian P,Hei Y,Wang R,Wang T,Yang J,Li J,Di Z,Liu Z,Baskys A,Liu W,Wu S,Long Q

Mon Aug 31 00:00:00 UTC 2020

Immunity against the Obligate Intracellular Bacterial Pathogen Rickettsia australis Requires a Functional Complement System.

Infection and immunity

Riley SP,Fish AI,Del Piero F,Martinez JJ

PA5-21349 was used in Flow cytometry/Cell sorting to look into when the complement system is activated during infection with Rickettsia australis in mice and how immunity can be drawn from this.

Fri Jun 01 00:00:00 UTC 2018

生物信息学

蛋白别名: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3

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基因别名: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

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UniProt ID: (Human) P01024, (Mouse) P01027

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Entrez Gene ID: (Human) 718, (Mouse) 12266

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功能

serine-type endopeptidase activity endopeptidase inhibitor activity receptor binding protein binding lipid binding C5L2 anaphylatoxin chemotactic receptor binding cofactor binding protease inhibitor

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