Invitrogen

Complement C3b Monoclonal Antibody (6C9)

Complement C3b Antibody in Flow Cytometry (Flow)

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Complement C3b Antibody (MA1-70053) in Flow

Flow cytometric analysis of C57BL/6 mouse thymocytes incubated with rabbit anti-mouse T cells and then incubated with fresh human serum were stained using a Complement C3b monoclonal antibody (Product # MA1-70053) (filled histogram) or mouse IgG1 isotype control (open histogram).

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产品信息

MA1-70053

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/1 million cells

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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功能检测 (Functional)

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6C9

抗原

Human C3.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_1073823

产品详细信息

MA1-70053 detects C3/C3b/iC3b in human and mouse samples.

MA1-70053 has been successfully used in flow cytometry and ELISA procedures.

The MA1-70053 immunogen is human C3.

Store at 4ºC. For long term storage, aliquot and freeze unused portion at -20ºC in volumes appropriat for single usage. Avoid freeze/thaw cycles.

靶标信息

Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb.

Blood advances

Rajagopal V,Leksa N,Gorham R,Jindal S,Nair S,Knockenhauer K,Chan J,Byun T,Mercadante C,Moore S,Panicker S,Parry G,Storek M

Published figure using Complement C3b monoclonal antibody (Product # MA1-70053) in Flow Cytometry

Tue Aug 22 00:00:00 UTC 2023

Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria.

Frontiers in cellular and infection microbiology

Sun Y,Xu B,Zhuge X,Tang F,Wang X,Gong Q,Chen R,Xue F,Dai J

MA1-70053 was used in Functional Assay to conclude that the identification of novel outer membrane-displayed CMEs which played a role in the FH recruitment contributes to the elucidation of the molecular mechanism of ExPEC pathogenicity.

Thu Jun 17 00:00:00 UTC 2021

All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-interleukin-1β loop.

Haematologica

Zhu X,Wang Y,Jiang Q,Jiang H,Lu J,Wang Y,Kong Y,Chang Y,Xu L,Peng J,Hou M,Huang X,Zhang X

MA1-70053 was used in an ELISA assay to study the role of the complement-IL-1Β loop in the impaired bone marrow microenvironment.

Thu Aug 01 00:00:00 UTC 2019

TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins.

Blood

Shi J,Rose EL,Singh A,Hussain S,Stagliano NE,Parry GC,Panicker S

MA1-70053 was used in flow cytometry to study the efficacy of the serine protease C1s inhibitor TNT003 in the treatment of cold agglutinin disease

Thu Jun 26 00:00:00 UTC 2014

生物信息学

蛋白别名: Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; iC3b; prepro-C3

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基因别名: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

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UniProt ID: (Human) P01024, (Mouse) P01027

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Entrez Gene ID: (Human) 718, (Mouse) 12266

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功能

serine-type endopeptidase activity endopeptidase inhibitor activity receptor binding protein binding lipid binding C5L2 anaphylatoxin chemotactic receptor binding cofactor binding

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