Invitrogen

Connexin 30.2 Polyclonal Antibody

Connexin 30.2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Connexin 30.2 Antibody (40-7400) in IHC (P)

Immunohistochemistry analysis of Connexin 30.2 showing staining in the membrane and cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at ... View More

Connexin 30.2 Antibody in Immunohistochemistry (IHC)

Connexin 30.2 Antibody in Western Blot (WB)

Connexin 30.2 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))

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产品信息

40-7400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

2-4 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the c-term region of the mouse connexin 30.2 protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533480

产品详细信息

40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.

靶标信息

Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Dual Roles of Triiodothyronine in Regulating the Morphology of Hair Cells and Supporting Cells during Critical Periods of Mouse Cochlear Development.

International journal of molecular sciences

Bai X,Xu K,Xie L,Qiu Y,Chen S,Sun Y

40-7400 was used in Immunohistochemistry to increase the reserve of supporting cells.

Sat Feb 25 00:00:00 UTC 2023

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

40-7400 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

40-7400 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

40-7400 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers.

Cancers

Pedrosa RMSM,Wismans LV,Sinke R,van der Weiden M,van Eijck CHJ,Kros JM,Mustafa DAM

40-7400 was used in Immunohistochemistry to identify genes involved in the formation of brain metastasis independently from the T cell response.

Tue Jun 15 00:00:00 UTC 2021

Cardiomyocyte Expression of ZO-1 Is Essential for Normal Atrioventricular Conduction but Does Not Alter Ventricular Function.

Circulation research

Zhang J,Vincent KP,Peter AK,Klos M,Cheng H,Huang SM,Towne JK,Ferng D,Gu Y,Dalton ND,Chan Y,Li R,Peterson KL,Chen J,McCulloch AD,Knowlton KU,Ross RS

40-7400 was used in Western Blot to determine the function of cardiac myocyte ZO-1 (Zonula occludens-1) in the intact heart, given its binding to other cardiac myocyte proteins involved in normal cardiac conduction and function.

Fri Jul 03 00:00:00 UTC 2020

Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition.

Scientific reports

Bhattacharyya S,Duan J,Wang L,Li B,Bhakta M,Fernandez-Perez A,Hon GC,Munshi NV

40-7400 was used in Immunohistochemistry-immunofluorescence to develop a novel Gjd3-CreEGFP mouse line which successfully recombines floxed alleles within the atrioventricular node beginning at E12.5.

Thu Feb 14 00:00:00 UTC 2019

Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.

Biochemical and biophysical research communications

Gemel J,Lin X,Collins R,Veenstra RD,Beyer EC

Fri May 02 00:00:00 UTC 2008

Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.

Biochemical and biophysical research communications

Gemel J,Lin X,Collins R,Veenstra RD,Beyer EC

Fri May 02 00:00:00 UTC 2008

Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.

Biochemical and biophysical research communications

Gemel J,Lin X,Collins R,Veenstra RD,Beyer EC

Fri May 02 00:00:00 UTC 2008

Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.

Biochemical and biophysical research communications

Gemel J,Lin X,Collins R,Veenstra RD,Beyer EC

Fri May 02 00:00:00 UTC 2008

生物信息学

蛋白别名: connexin 30.2; connexin 31.9; Connexin-30.2; Connexin-31.9; Cx30.2; Cx31.9; Gap junction alpha-11 protein; Gap junction chi-1 protein; Gap junction delta-3 protein; gap junction membrane channel protein chi 1; gap junction protein, delta 3, 31.9kDa

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基因别名: Cx30.2; CX31.9; GJA11; GJC1; GJD3

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UniProt ID: (Human) Q8N144, (Mouse) Q80ZH0

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Entrez Gene ID: (Human) 125111, (Mouse) 353155

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功能

ion channel activity gap junction channel activity protein binding gap junction

参与通路

cell communication response to glucose gap junction assembly ion transmembrane transport transmembrane transport