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Connexin 30 Antibody (700258) in ICC/IF
Immunofluorescent analysis of Connexin 30 was performed on 90% confluent log phase Caco-2 cells The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 30 Recombinant Rabbit Monoclonal Antibody (Product # 700258) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled wi... View More
产品信息
700258
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
16H9L8
抗原
A peptide corresponding to amino acids 241-261 of P70689.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532309
产品详细信息
This antibody is predicted to react with human, rat, hamster and bovine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: connexin 30; Connexin-30; Cx30; ectodermal dysplasia 2, hidrotic (Clouston syndrome); Gap junction beta-6 protein; gap junction membrane channel protein beta 6; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6, 30kDa
基因别名: AA958971; CX30; Cxn-30; D14Bwg0506e; DFNA3; DFNA3B; DFNB1B; ECTD2; ED2; EDH; GJB6; HED; HED2
UniProt ID: (Human) O95452, (Mouse) P70689
Entrez Gene ID: (Human) 10804, (Mouse) 14623
