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Connexin 31 Antibody (36-5100) in ICC/IF
Immunofluorescence analysis of CONNEXIN 31 was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Connexin 31 Rabbit Polyclonal Antibody (Product # 36-5100) at 2µg/mL in 0.1% BSA and incubated for 3 hours a... View More
产品信息
36-5100
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from the C-terminal region of mouse connexin 31 protein
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533265
产品详细信息
This antibody reacts with the ~32 kDa mouse Connexin 31 on Western blots; the band observed at ~62 kDa is probably due to dimerization of Connexin 31. Several higher bands of unknown origin are observed in western blots of mouse skin homogenates.
靶标信息
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: connexin 31; Connexin-31; Cx31; Gap junction beta-3 protein; gap junction membrane channel protein beta 3; gap junction protein, beta 3, 31kDa
基因别名: Cnx31; CX31; Cxn-31; D4Wsu144e; DFNA2; DFNA2B; EKV; Gjb-3; GJB3
UniProt ID: (Human) O75712, (Mouse) P28231
Entrez Gene ID: (Human) 2707, (Mouse) 14620
