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Connexin 43 Polyclonal Antibody

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说明书
实验方案
技术支持

产品信息

600-401-MJ2

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
-

免疫组化 (IHC)

1:200
-

免疫细胞化学 (ICC/IF)

15 µg/mL
-

酶联免疫吸附实验 (ELISA)

1:10,000-1:15,000
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.92 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against rat connexin 43. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat, black bear, European rabbit, wild boar, Macaca fascicularis, and Chlorocebus aethiops.

靶标信息

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; ODD; ODOD; SDTY3; Syndactyly type III

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基因别名: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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功能
signal transducer activity receptor binding gap junction channel activity protein binding ion transmembrane transporter activity SH3 domain binding PDZ domain binding beta-tubulin binding connexin binding gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling protein domain specific binding transmembrane transporter activity gap junction
参与通路
osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping regulation of heart rate epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration endothelium development atrial ventricular junction remodeling transport apoptotic process muscle contraction signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling heart development adult heart development response to pH response to glucose vascular transport positive regulation of gene expression negative regulation of gene expression cell communication by chemical coupling cell communication by electrical coupling positive regulation of cell communication by chemical coupling ATP transport gap junction assembly negative regulation of cell growth regulation of bone mineralization positive regulation of insulin secretion ion transmembrane transport response to fluid shear stress embryonic digit morphogenesis positive regulation of I-kappaB kinase/NF-kappaB signaling skeletal muscle tissue regeneration response to peptide hormone positive regulation of osteoblast differentiation positive regulation of protein catabolic process positive regulation of striated muscle tissue development positive regulation of vasoconstriction positive regulation of vasodilation regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis protein oligomerization regulation of calcium ion transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization negative regulation of wound healing cellular response to mechanical stimulus atrial cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction negative regulation of DNA biosynthetic process regulation of bicellular tight junction assembly positive regulation of behavioral fear response epicardial cell to mesenchymal cell transition cell communication regulation of heart contraction negative regulation of cell proliferation embryonic heart tube development cell-cell junction organization transmembrane transport regulation of blood vessel remodeling cardiac conduction establishment of epithelial cell polarity
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