Invitrogen

Connexin 50 Monoclonal Antibody (C6)

Connexin 50 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

Connexin 50 Antibody (33-4300) in ICC/IF

Immunofluorescent analysis of Connexin 50 was done on 90% confluent log phase CaCo-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at... View More

Connexin 50 Antibody in Western Blot (WB)

Connexin 50 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

产品信息

33-4300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Sheep, Rat

已发表种属

Human, Mouse, Pig, Rabbit, Rat

宿主/亚型

Mouse / IgM, kappa

分类

Monoclonal

类型

Antibody

克隆号

抗原

This antibody was prepared against urea/alkali stripped sheep lens membranes.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533120

靶标信息

This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fabbiani G,Reali C,Valentín-Kahan A,Rehermann MI,Fagetti J,Falco MV,Russo RE

33-4300 was used in Immunohistochemistry to study the role of connexin signalling in the central canal in the reactivation of latent stem cell after spinal cord injury.

Wed Mar 11 00:00:00 UTC 2020

Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes.

American journal of physiology. Endocrinology and metabolism

Pelletier RM,Akpovi CD,Chen L,Vitale ML

33-4300 was used in Western Blotting to indicate that the db mutation-induced impairment of meiosis may arise from imbalances in cholesterol metabolism and upregulated Cx43 expression and phosphorylation in tubules.

Mon Jan 01 00:00:00 UTC 2018

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.

Cell and tissue research

Rodriguez-Jimenez FJ,Alastrue A,Stojkovic M,Erceg S,Moreno-Manzano V

33-4300 was used in immunocytochemistry and western blot to investigate modulation of Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells by connexin 50

Mon Aug 01 00:00:00 UTC 2016

Expression and Localization of Connexins in the Outer Retina of the Mouse.

Journal of molecular neuroscience : MN

Bolte P,Herrling R,Dorgau B,Schultz K,Feigenspan A,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunohistochemistry (frozen) to investigate the counterpart of connexin36 at gap junctions between rods and cones.

Mon Feb 01 00:00:00 UTC 2016

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 UTC 2012

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 UTC 2012

PKCγ, role in lens differentiation and gap junction coupling.

Current eye research

Das S,Wang H,Molina SA,Martinez-Wittinghan FJ,Jena S,Bossmann LK,Miller KA,Mathias RT,Takemoto DJ

33-4300 was used in western blot to use knock out mice to determine the role of PKCgamma in the regulation of gap junction coupling in the normal lens.

Fri Jul 01 00:00:00 UTC 2011

Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas.

Annals of the New York Academy of Sciences

Willenberg HS,Schott M,Saeger W,Tries A,Scherbaum WA,Bornstein SR

33-4300 was used in immunohistochemistry - paraffin section to examine connexin expression in chromaffin cells of normal human adrenal glands and benign and malignant pheochromocytomas

Tue Aug 01 00:00:00 UTC 2006

Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia.

Visual neuroscience

Zahs KR,Ceelen PW

33-4300 was used in immunohistochemistry - frozen section to study connexins in mammalian Muller cells

Mon Jun 05 00:00:00 UTC 2006

Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels.

Investigative ophthalmology & visual science

Zampighi GA,Planells AM,Lin D,Takemoto D

Thu Sep 01 00:00:00 UTC 2005

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 UTC 2004

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 UTC 2004

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 UTC 2001

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 UTC 2001

生物信息学

蛋白别名: alpha 8 connexin; cell surface glycoprotein; connexin 50; Connexin-49; Connexin-50; Cx49; Cx50; gap junction alpha 8; Gap junction alpha-8 protein; gap junction membrane channel protein alpha 8; gap junction membrane channel protein alpha-8; gap junction protein alpha 8 50kDa; gap junction protein, alpha 8, 50kDa; Lens fiber protein MP70; lens intrinsic membrane protein MP70; lens opacity 10; MP38; MP64

查看更多收起

基因别名: Aey5; CAE; CAE1; Cnx50; CTRCT1; CX50; CZP1; GJA8; Lop10; MP70

查看更多收起

UniProt ID: (Sheep) P55917, (Human) P48165, (Mouse) P28236

查看更多收起

Entrez Gene ID: (Sheep) 100170231, (Human) 2703, (Rat) 29601, (Mouse) 14616

查看更多收起

功能

gap junction channel activity channel activity protein binding gap junction

参与通路

lens development in camera-type eye transport cell-cell signaling visual perception protein homooligomerization transmembrane transport camera-type eye development cell communication