Invitrogen

CrkL Polyclonal Antibody

查看其他27个CrkL抗体

抗体检测数据 (4)

CrkL Antibody in Immunocytochemistry (ICC/IF)

CrkL Antibody in Immunocytochemistry (ICC/IF)

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CrkL Antibody (PA1-9033) in ICC/IF

Immunocytochemistry analysis of CrkL using CrkL Polyclonal Antibody (Product # PA1-9033) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

CrkL Antibody in Immunocytochemistry (ICC/IF)

CrkL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CrkL Antibody in Western Blot (WB)

CrkL Antibody in Flow Cytometry (Flow)

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CrkL Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CrkL using CrkL Polyclonal Antibody (Product # PA1-9033) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

CrkL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CrkL in human thymus. Samples were incubated with CrkL polyclonal antibody (Product # PA1-9033) using a dilution of 2.5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

CrkL Antibody in Western Blot (WB) — Western blot analysis of CrkL using CrkL Polyclonal Antibody (Product # PA1-9033) (0.1 µg/mL) in staining of K562 (A) and (1 µg/mL) Daudi (B) and NIH3T3 (C) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

CrkL Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of CrkL using CrkL Polyclonal Antibody (Product # PA1-9033) in paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

产品信息

PA1-9033

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-1 µg/mL

-

免疫组化（石蜡） (IHC (P))

2.5-3.8 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the C terminus amino acids KIFDPQNPDENE (aa 292-303).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2245352

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

靶标信息

May mediate the transduction of intracellular signals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Crk-like protein; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like

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基因别名: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol

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UniProt ID: (Human) P46109, (Mouse) P47941

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Entrez Gene ID: (Human) 1399, (Mouse) 12929

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signal transducer activity SH3/SH2 adaptor activity protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion

blood vessel development pattern specification process heart development organ morphogenesis anterior/posterior pattern specification thymus development parathyroid gland development activation of MAPKK activity JNK cascade Ras protein signal transduction positive regulation of cell proliferation intracellular signal transduction cell-cell adhesion positive regulation of substrate adhesion-dependent cell spreading