Invitrogen

CrkL Polyclonal Antibody

CrkL Antibody in Immunocytochemistry (ICC/IF)

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CrkL Antibody (PA5-28622) in ICC/IF

Immunofluorescence analysis of Crk-like protein was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CrkL Polyclonal Antibody (Product # PA5-28622) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More

CrkL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CrkL Antibody in Immunoprecipitation (IP)

CrkL Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CrkL Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

CrkL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SH-SY5Y cells were transfected with Crk-like protein siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CrkL Polyclonal Antibody (Product # PA5-28622). {KD}

产品信息

PA5-28622

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

1:100-1:500

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 282 of Human CrkL

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.33 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546098

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, U2OS.

Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (81%), Xenopus laevis (87%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

May mediate the transduction of intracellular signals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles.

mSphere

Carpenter V,Chen YS,Dolat L,Valdivia RH

Published figure using CrkL polyclonal antibody (Product # PA5-28622) in Immunoprecipitation

Wed Nov 20 00:00:00 UTC 2019

The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles.

mSphere

Carpenter V,Chen YS,Dolat L,Valdivia RH

Published figure using CrkL polyclonal antibody (Product # PA5-28622) in Immunoprecipitation

Wed Nov 20 00:00:00 UTC 2019

The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles.

mSphere

Carpenter V,Chen YS,Dolat L,Valdivia RH

Published figure using CrkL polyclonal antibody (Product # PA5-28622) in Immunoprecipitation

Wed Nov 20 00:00:00 UTC 2019

The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles.

mSphere

Carpenter V,Chen YS,Dolat L,Valdivia RH

Published figure using CrkL polyclonal antibody (Product # PA5-28622) in Immunoprecipitation

Wed Nov 20 00:00:00 UTC 2019

生物信息学

蛋白别名: Crk-like protein; crk-like protein {ECO:0000250|UniProtKB:P46109}; crkl {ECO:0000312|EMBL:AAH85865.1, ECO:0000312|RGD:1308531}; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog-like

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基因别名: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol

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UniProt ID: (Human) P46109, (Mouse) P47941, (Rat) Q5U2U2

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Entrez Gene ID: (Human) 1399, (Mouse) 12929, (Rat) 287942

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功能

signal transducer activity SH3/SH2 adaptor activity protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

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