Invitrogen

Cullin 4A Polyclonal Antibody

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抗体检测数据 (2)
高级验证 (1)

Cullin 4A Antibody in Western Blot (WB)

Cullin 4A Antibody in Western Blot (WB)

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Cullin 4A Antibody (PA5-17101) in WB

Western blot was performed using Anti-Cullin 4A Polyclonal Antibody (Product # PA5-17101). An 87 kDa band along with a 77 kDa band, which is a reported isoform corresponding to CUL4A was observed across cell lines tested and increased upon TGF-beta 1 treatment in A549. Modified whole cell extracts (1% SDS) (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), A549 (Lane 3), A549 treated with TGF-beta 1 (10 ng/mL for 24 hr) (Lane 4), LNCaP (Lane 5), HeLa (Lane 6) and Hep G2 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris... View More

Cullin 4A Antibody in Western Blot (WB)

Cullin 4A Antibody in Western Blot (WB)

Cullin 4A Antibody

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Cullin 4A Antibody in Western Blot (WB) — Western blot was performed using Anti-Cullin 4A Polyclonal Antibody (Product # PA5-17101). An 87 kDa band along with a 77 kDa band, which is a reported isoform corresponding to CUL4A was observed across cell lines tested and increased upon TGF-beta 1 treatment in A549. Modified whole cell extracts (1% SDS) (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), A549 (Lane 3), A549 treated with TGF-beta 1 (10 ng/mL for 24 hr) (Lane 4), LNCaP (Lane 5), HeLa (Lane 6) and Hep G2 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Cullin 4A Antibody in Western Blot (WB) — Western blot analysis of CUL4A in extracts from COS and SK-MEL-28 cell lines using CUL4A polyclonal antibody (Product # PA5-17101).

Cullin 4A Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Cullin 4A Polyclonal Antibody (Product # PA5-17101), shows increased expression of CUL4A in A549 upon TGF-beta1 treatment. {TM}

产品信息

PA5-17101

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫沉淀 (IP)

1:50

-

产品规格

种属反应

Human, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding residues surrounding Ser12 of human CUL4A

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

16 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10978708

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CUL 4A; CUL-4A; Cul4a protein; Cullin-4A; MGC36573; MGC64071; OTTHUMP00000018753

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基因别名: CUL4A

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UniProt ID: (Human) Q13619

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Entrez Gene ID: (Human) 8451

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protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein ligase

G1/S transition of mitotic cell cycle nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion cellular response to DNA damage stimulus cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation viral process hemopoiesis negative regulation of granulocyte differentiation nucleotide-excision repair, DNA incision somatic stem cell population maintenance DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein metabolic process global genome nucleotide-excision repair intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle regulation of DNA damage checkpoint regulation of nucleotide-excision repair