Invitrogen

Cullin 4A Polyclonal Antibody

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抗体检测数据 (2)

Cullin 4A Antibody in Western Blot (WB)

Cullin 4A Antibody in Western Blot (WB)

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Cullin 4A Antibody (PA5-96216) in WB

Western blot analysis of Cullin 4A in extracts of various cell lines. Samples were incubated with Cullin 4A Polyclonal antibody (Product # PA5-96216) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Cullin 4A Antibody in Western Blot (WB)

Cullin 4A Antibody in Immunoprecipitation (IP)

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Cullin 4A Antibody in Western Blot (WB) — Western blot analysis of Cullin 4A in extracts of various cell lines. Samples were incubated with Cullin 4A Polyclonal antibody (Product # PA5-96216) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Cullin 4A Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Cullin 4A in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg Cullin 4A Polyclonal antibody (Product # PA5-96216). Western blot was performed from the immunoprecipitate using Cullin 4A Polyclonal antibody (Product # PA5-96216) at a dilution of 1:1,000.

产品信息

PA5-96216

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

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免疫细胞化学 (ICC/IF)

1:50-1:100

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酶联免疫吸附实验 (ELISA)

1 µg/mL

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免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-300 of human Cullin 4A (NP_0010088951)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.43 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808018

产品详细信息

Positive Samples: K-562, BT-474, HeLa, Mouse testis

Immunogen sequence: MADEAPRKGS FSALVGRTNG LTKPAALAAA PAKPGGAGGS KKLVIKNFRD RPRLPDNYTQ DTWRKLHEAV RAVQSSTSIR YNLEELYQAV ENLCSHKVSP MLYKQLRQAC EDHVQAQILP FREDSLDSVL FLKKINTCWQ DHCRQMIMIR SIFLFLDRTY VLQNSTLPSI WDMGLELFRT HIISDKMVQS KTIDGILLLI ERERSGEAVD RSLLRSLLGM LSDLQVYKDS FELKFLEETN CLYAAEGQRL MQEREVPEYL NHVSKRLEEE GDRVITYLDH STQKPLIACV EKQLLGEHLT

靶标信息

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CUL 4A; CUL-4A; Cul4a protein; Cullin-4A; MGC36573; MGC64071; OTTHUMP00000018753

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基因别名: 2810470J21Rik; AW495282; CUL4A; RGD1563853

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UniProt ID: (Human) Q13619, (Mouse) Q3TCH7

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Entrez Gene ID: (Human) 8451, (Mouse) 99375, (Rat) 361181

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protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein ligase

G1/S transition of mitotic cell cycle nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion cellular response to DNA damage stimulus cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation viral process hemopoiesis negative regulation of granulocyte differentiation nucleotide-excision repair, DNA incision somatic stem cell population maintenance DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein metabolic process global genome nucleotide-excision repair intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle regulation of DNA damage checkpoint regulation of nucleotide-excision repair DNA repair ubiquitin-dependent protein catabolic process positive regulation of protein catabolic process