Invitrogen

Cullin 4B Polyclonal Antibody

Cullin 4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cullin 4B Antibody (PA5-119728) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat cerebellum tissue using Cullin 4B Polyclonal Antibody (Product # PA5-119728). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Cullin 4B antibody at a d... View More

Cullin 4B Antibody in Flow Cytometry (Flow)

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产品信息

PA5-119728

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within human Cullin 4B aa 850-913

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913301

产品详细信息

Positive Control: HUVEC cell lysates, rat cerebellum tissue, human colon tissue, mouse testis tissue, Daudi.

Subcellular Location: Nucleus.

靶标信息

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

仅用于科研。不用于诊断过程。未经明确授权不得转售。