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Cyclin A Antibody (PA5-99161) in IHC (P)
Immunohistochemistry analysis of Cyclin A in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Cyclin A polyclonal antibody (Product # PA5-99161) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99161
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CCNA1(Accession P78396), corresponding to amino acid residues L416-Q465.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818094
Product Specific Information
Antibody detects endogenous levels of total Cyclin A.
Target Information
The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin A; Cyclin A2 (Cyclin A); Cyclin-A; Cyclin-A1; Cyclin-A2; CyclinA; CyclinA1; testicular tissue protein Li 34
Gene Aliases: AA408589; Ccn-1; CCN1; CCNA; CCNA1; CCNA2; CT146; Cyca; CycA2
UniProt ID: (Human) P78396, (Human) P20248, (Rat) Q6AY13, (Mouse) Q61456, (Mouse) P51943
Entrez Gene ID: (Human) 8900, (Human) 890, (Rat) 295052, (Mouse) 12427, (Rat) 114494, (Mouse) 12428
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
mitotic nuclear division
male meiosis I
spermatogenesis
cell division
regulation of cyclin-dependent protein serine/threonine kinase activity
mitotic G2 DNA damage checkpoint
Ras protein signal transduction
viral process
organ regeneration
response to glucagon
cellular response to platelet-derived growth factor stimulus
cellular response to leptin stimulus
positive regulation of transcription, DNA-templated
positive regulation of fibroblast proliferation
cellular response to cocaine
cellular response to luteinizing hormone stimulus
cellular response to estradiol stimulus
cellular response to hypoxia
cellular response to nitric oxide
cochlea development
cellular response to insulin-like growth factor stimulus
regulation of G2/M transition of mitotic cell cycle
cell cycle
multicellular organism development
cell differentiation
meiotic cell cycle
response to estradiol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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