Invitrogen

Cyclin B2 Monoclonal Antibody (X29.2)

Cyclin B2 Antibody in Immunocytochemistry (ICC/IF)

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Cyclin B2 Antibody (MA1-156) in ICC/IF

Immunofluorescence analysis of Cyclin B2 was performed using 70% confluent log phase HeLa cells treated with Camptothecin (100nM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin B2 Monoclonal Antibody (Product # MA1-156) at 1:100 dilution in ... View More

Cyclin B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclin B2 Antibody in Immunoprecipitation (IP)

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产品信息

MA1-156

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:20-1:200

免疫沉淀 (IP)

1:1,000

产品规格

种属反应

Human, Mouse, Non-human primate, Xenopus

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

X29.2

抗原

Xenopus laevis cyclin B2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536864

产品详细信息

MA1-156 detects Cyclin B2 in mammalian samples. MA1-156 has been successfully used in Immunofluorescence, Immunoprecipitation, IHC (P) and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-156 show the accumulation of a prominent band at ~51 kDa in camptothecin and hydroxyurea treated cells. MA1-156 also detects additional unknown band at ~80 kDa. In Immunofluorescence applications, MA1-156 shows cyclin B2 staining consistent with the Golgi region, whereas MA1-155 shows cyclin B1 co-localization with microtubules.

MA1-156 reacts with Cyclin B2 from Xenopus laevis and mammalian sources. In Western blot applications, X29.2 also cross reacts with Cyclin B1.

靶标信息

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

仅用于科研。不用于诊断过程。未经明确授权不得转售。