Invitrogen

Cyclin D2 Polyclonal Antibody

Cyclin D2 Antibody in Immunocytochemistry (ICC/IF)

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Cyclin D2 Antibody (PA5-97550) in ICC/IF

Immunofluorescent analysis of Cyclin D2 in HepG2 cells using a Cyclin D2 polyclonal antibody (Product # PA5-97550) at a dilution of 1:75. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells we... View More

Cyclin D2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-97550

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

免疫组化（石蜡） (IHC (P))

1:20-1:500

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human G1/S-specific cyclin-D2 protein (1-289AA)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.58 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2812166

靶标信息

Cyclin D2 is a G1 cyclin required for G1 phase progression and is a strong candidate for a proto-oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。