NeoBiotechnologies

Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS, 3128R)

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

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Cytochrome C Antibody (54205-RBM6-P1ABX) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1ABX) followed by goat anti-rabbit IgG-CF488 (Green).

Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytochrome C Antibody in Western Blot (WB)

Cytochrome C Antibody in Flow Cytometry (Flow)

Cytochrome C Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

54205-RBM6-P1ABX

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

0.25-0.5 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

产品规格

种属反应

Amphibian, Avian, Dog, Fruit fly, Horse, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

CYCS, 3128R

抗原

Recombinant full-length human CYCS protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CG17903-PA; CG17903-PB; CRF; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p

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基因别名: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4