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Cytohesin 1/2 Antibody (MA1-062) in ICC/IF
Immunofluorescent analysis of Cytohesin 1, 2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1, 2 monoclonal antibody (Product # MA1-062) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 1, 2 staining (... View More
产品信息
MA1-062
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
6G11
抗原
Purified, His-tagged cytohesin-1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
PEG precipitation
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2089016
产品详细信息
MA1-062 detects recombinant human and mouse cytohesin-1 and 2 (ARNO).
MA1-062 has been successfully used in Western blot, IF and immunoprecipitation procedures. By Western blot, this antibody detects an ~48 kDa band representing both cytohesin-1 and cytohesin-2. In immunoprecipitation procedures MA1-062 only precipitates cytohesin-2.
The MA1-062 immunogen is purified, His-tagged cytohesin-1.
靶标信息
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ARF exchange factor; ARF nucleotide-binding site opener; B2-1/ARNO; CLM1; CLM2; CTC-273B12.8; cytoadhesin 1; Cytohesin-1; Cytohesin-2; homolog of secretory protein SEC7; mSec7-1; mSec7-2; PH, SEC7 and coiled-coil domain-containing protein 1; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 1; pleckstrin homology, Sec7 and coiled-coil domains 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2); Protein ARNO; SEC7 homolog A; SEC7 homolog B; SEC7 homolog B2-1
基因别名: ARNO; B2-1; CLM1; CLM2; CTH-1; CTS18; CTS18.1; CYTH1; CYTH2; CYTIP; CYTOHESIN-1; D17S811E; mKIAA4240; PSCD1; PSCD2; PSCD2L; SEC7; Sec7b; SEC7L; Sec7p-L; Sec7p-like
UniProt ID: (Human) Q9P123, (Human) Q99418, (Mouse) Q9QX11, (Mouse) P63034
Entrez Gene ID: (Human) 9267, (Human) 9266, (Mouse) 19157, (Mouse) 19158
