Invitrogen

Cytokeratin 18 Monoclonal Antibody (LDK18), Alexa Fluor™ 488, eBioscience™

Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)

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Cytokeratin 18 Antibody (53-9815-82) in ICC/IF

Immunocytochemistry of fixed MCF-7 cells stained with 5 µg/mL Mouse IgG1 K Isotype Control Alexa Fluor® 488 (left) or 5 µg/mL Anti-Human Cytokeratin 18 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

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产品信息

53-9815-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-Dependent

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免疫细胞化学 (ICC/IF)

5 µg/mL

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流式细胞分析 (Flow)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

LDK18

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574481

产品详细信息

Description: The monoclonal antibody LDK18 recognizes human Cytokeratin 18. Cytokeratin 18 is member of the large family of intermediate filaments found within epithelial cells. The family of cytokeratins is made up of type I (acidic) and type II (basic) members which form heterodimers by pairing an acidic with a basic member to compose the keratin filament. Cytokeratin 18 (type I) is most commonly found associated with cytokeratin 8 (type II). Because many tumors of epithelial origin retain expression of keratin proteins, cytokeratin 18 has been used as a tumor marker to identify both tumors and metastases. In addition, cytokeratin 18 has been shown to be expressed in highly proliferative retinal pigment epithelium cells.

Applications Reported: This LDK18 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This LDK18 antibody has been tested by immunocytochemistry of MeOH-fixed human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts.

In vitro models

Jones S,Ashworth JC,Meakin M,Collier P,Probert C,Ritchie AA,Merry CLR,Grabowska AM

53-9815-82 was used in Immunohistochemistry to demonstrate the ;ltential of hydrogel as an breast cancer model for the passage of patient-derived xenografts (PDXs).

Tue Oct 31 00:00:00 UTC 2023

High‑throughput and continuous flow isolation of rare circulating tumor cells and clusters in gastric cancer from human whole blood samples using electromagnetic vibration‑based filtration.

Oncology reports

Xiang A,Xue M,Ren F,Wang L,Ye Z,Li D,Ji Q,Ji G,Lu Z

53-9815-82 was used in Immunocytochemistry to demonstrate the eVBF device that efficiently isolates rare CTCs and CTC clusters from clinical blood samples of patients with gastric cancer.

Mon Jun 01 00:00:00 UTC 2020

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

53-9815 was used in Flow cytometry/Cell sorting to test the hypothesis that integrins are pro-fibrotic via regulation of functional interactions between tubular epithelial cells and renal fibroblasts.

Tue Sep 01 00:00:00 UTC 2020

An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix.

Biology open

Qureshi OS,Bon H,Twomey B,Holdsworth G,Ford K,Bergin M,Huang L,Muzylak M,Healy LJ,Hurdowar V,Johnson TS

53-9815 was used in Flow cytometry/Cell sorting to explore, through an immunofluorescence assay, the role of human epithelial cells in fibrillar extracellular matrix production.

Sun Oct 15 00:00:00 UTC 2017

An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix.

Biology open

Qureshi OS,Bon H,Twomey B,Holdsworth G,Ford K,Bergin M,Huang L,Muzylak M,Healy LJ,Hurdowar V,Johnson TS

53-9815 was used in Flow cytometry/Cell sorting to explore, through an immunofluorescence assay, the role of human epithelial cells in fibrillar extracellular matrix production.

Sun Oct 15 00:00:00 UTC 2017

生物信息学

蛋白别名: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin-18; Cytokeratin18; K 18; K18; keratin 18, type I; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

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基因别名: CK-18; CYK18; K18; KRT18; PIG46

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UniProt ID: (Human) P05783

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Entrez Gene ID: (Human) 3875

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功能

structural molecule activity protein binding poly(A) RNA binding scaffold protein binding cadherin binding involved in cell-cell adhesion

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