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Cytokeratin 20 Antibody (PA5-116446) in ICC/IF
Immunocytochemical analysis of Cytokeratin 20 in SW480 cells using a Cytokeratin 20 polyclonal antibody (Product #PA5-116446). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed... View More
产品信息
PA5-116446
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within Human Cytokertin 20 aa 375-424
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2901078
产品详细信息
Positive controls: Human small intestine tissue lysates, mouse colon tissue lysates, SW480, human colon tissue, Human small intestine tissue, mouse colon tissue, mouse small intestine tissue, JAR.
靶标信息
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CK-20; CK-21; cytokeratin 20; cytokeratin 21; Cytokeratin-20; Cytokeratin-21; K20; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; krt20; MGC35423; Protein IT
基因别名: 9030623C06Rik; CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900, (Mouse) Q9D312, (Rat) P25030
Entrez Gene ID: (Human) 54474, (Mouse) 66809, (Rat) 286912
