Invitrogen

Cytokeratin 8 Polyclonal Antibody

Cytokeratin 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cytokeratin 8 Antibody (PA5-29607) in IHC (P)

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8 (Product # PA5-29607) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Cytokeratin 8 Antibody in Western Blot (WB)

Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

PA5-29607

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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体外检测 (IV)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 49 and 301 of Human Cytokeratin 8

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.12 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547083

产品详细信息

Recommended positive controls: HepG2, HeLa, mouse liver, rat liver.

Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (83%), Xenopus laevis (85%), Dog (91%), Pig (91%), Rhesus Monkey (99%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

PA5-29607 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, In Vitro Assay to develop an advanced multiplexed immunostaining approach using SUM149-derived orthotopic tumors and find that the EMT state and epithelial-mesenchymal heterogeneity are predictive of overall survival in a cohort of stage III breast cancer.

Fri Aug 05 00:00:00 UTC 2022

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

Fri Aug 05 00:00:00 UTC 2022

Limiting Self-Renewal of the Basal Compartment by PKA Activation Induces Differentiation and Alters the Evolution of Mammary Tumors.

Developmental cell

Ognjenovic NB,Bagheri M,Mohamed GA,Xu K,Chen Y,Mohamed Saleem MA,Brown MS,Nagaraj SH,Muller KE,Gerber SA,Christensen BC,Pattabiraman DR

PA5-29607 was used in Immunohistochemistry to show that, by acting through the repression of SOX4, PKA activation promotes tumor differentiation and represents a possible adjuvant to chemotherapy for certain breast cancers.

Mon Dec 07 00:00:00 UTC 2020

Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish.

Frontiers in physiology

Pérez-Sánchez J,Terova G,Simó-Mirabet P,Rimoldi S,Folkedal O,Calduch-Giner JA,Olsen RE,Sitjà-Bobadilla A

Published figure using Cytokeratin 8 polyclonal antibody (Product # PA5-29607) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

生物信息学

蛋白别名: CK-8; cytokeratin 8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

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基因别名: AA960620; AL022697; AU019895; CARD2; CK-8; CK8; CYK8; CYKER; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; KRT8

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UniProt ID: (Human) P05787, (Mouse) P11679, (Rat) Q10758

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Entrez Gene ID: (Human) 3856, (Mouse) 16691, (Rat) 25626

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功能

structural molecule activity protein binding protein complex binding scaffold protein binding

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