NeoBiotechnologies

Cytokeratin 8 (KRT8) Recombinant Mouse Monoclonal Antibody (rKRT8, 4209)

Cytokeratin 8 (KRT8) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cytokeratin 8 (KRT8) Antibody (3856-MSM19-P1) in IHC (P)

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human brain using Cytokeratin 8 (KRT8) Recombinant Monoclonal Antibody (Product # 3856-MSM19-P1) at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. Secondary: HRP-polymer, 30 min. DAB, 5 min.

Cytokeratin 8 (KRT8) Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

3856-MSM19-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

rKRT8, 4209

抗原

Recombinant full-length human KRT8 protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

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