Invitrogen

Cytokeratin HMW Monoclonal Antibody (34BetaE12)

Cytokeratin HMW Antibody in Immunocytochemistry (ICC/IF)

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Cytokeratin HMW Antibody (MA5-12135) in ICC/IF

Immunofluorescence analysis of Cytokeratin HMW was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of Cytokeratin HMW (34BetaE12) Mouse Monoclonal Antibody (Product # MA5-12135) at 1:250 dilution in0.1% BSA and inc... View More

Cytokeratin HMW Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin HMW Antibody in Western Blot (WB)

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产品信息

MA5-12135

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化 (IHC)

查看13篇已发表文章 13篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:250

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Horse, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

34BetaE12

抗原

Solubilized keratin extracted from human stratum corneum

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_10983918

产品详细信息

MA5-12135 targets Cytokeratin HMW and it recognizes keratin 1, 5, 10, and 14 in the Moll catalog (MW 68kDa, 58kDa, 56.5kDa, and 50kDa), respectively. The antibody has been tested in ICC/IF, WB and IHC (P) applications and shows reactivity with Equine, Human, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications.

In normal cells, Cytokeratin HMW labels squamous, ductal and other complex epithelia. It reacts with benign small-acinir lesions of the prostate and does not react with hepatocytes, pancreatic acinar cells, proximal renal tubes or endometrial glands. In tumor cells, this antibody is reactive with both squamous and ductal neoplasms and variably with those derived from simple epithelium.

The MA5-12135 immunogen is solubilized keratin extracted from human stratum corneum.

靶标信息

Keratins are heteropolymeric structural proteins that form the intermediate filament. Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of cytokeratins - the low weight, acidic type I and the high weight, basic type II. Cytokeratins are usually found in pairs - one type I and one type II. KRT1 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase I (RACK1). In complex with C1QBP it is a high affinity receptor for kininogen-1/HMWK. KRT1 is expressed in the spinous and granular layers of the epidermis with family member KRT10. Mutations affecting the gene result in bullous congenital ichthyosiform erythroderma. KRT5 is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations affecting the gene result in epidermolysis bullosa simplex diseases. KRT10 plays a role in the establishment of the epidermal barrier on plantar skin. It also acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB - thus it plays a role in nasal colonization. Mutations affecting the gene result in epidermolytic hyperkeratosis. KRT14’s nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Mutations affecting the gene result in epidermolysis bullosa simplex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

MA5-12135 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 UTC 2022

Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients.

Cancer research communications

Jung M,Kowalczyk K,Hankins R,Bandi G,Kallakury B,Carrasquilla MA,Banerjee PP,Grindrod S,Dritschilo A

MA5-12135 was used in Immunocytochemistry-immunofluorescence to examine viability of cells following exposure to the antiandrogen (bicalutamide) and two PARP inhibitors (olaparib and niraparib) and observed decreased viability of tumor-derived cells as compared with viability of normal prostate-derived cells.

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Mechanisms of Endogenous HIV-1 Reactivation by Endocervical Epithelial Cells.

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Gornalusse GG,Valdez R,Fenkart G,Vojtech L,Fleming LM,Pandey U,Hughes SM,Levy CN,Dela Cruz EJ,Calienes FL,Kirby AC,Fialkow MF,Lentz GM,Wagoner J,Jing L,Koelle DM,Polyak SJ,Fredricks DN,McElrath MJ,Wald A,Hladik F

MA5-12135 was used in Immunohistochemistry (PFA fixed) to find that the columnar epithelial cells lining the endocervix, the lower part of the uterus, are particularly effective in reactivating HIV-1 from infected T cells.

Thu Apr 16 00:00:00 UTC 2020

Reevaluation of MAML2 fusion-negative mucoepidermoid carcinoma: a subgroup being actually hyalinizing clear cell carcinoma of the salivary gland with EWSR1 translocation.

Human pathology

Hsieh MS,Wang H,Lee YH,Ko JY,Chang YL

MA512135 was used in immunohistochemistry - paraffin section to use MAML2 staining to distinguish cases of hyalinizing clear cell carcinoma from mucoepidermoid carcinoma

Wed Mar 01 00:00:00 UTC 2017

Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S

MA5-12135 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population

Mon Apr 01 00:00:00 UTC 2013

Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Stefanović D,Stefanović M,Nikin Z

MA5-12135 was used in immunohistochemistry to develop a protocol for the counterstaining of immunohistochemical sections using Romanowsky-Giemsa

Thu Aug 01 00:00:00 UTC 2013

Sinonasal teratocarcinosarcoma: a clinical and pathological analysis.

International journal of surgical pathology

Yang S,Sun R,Liang J,Zhou Z,Zhou J,Rui J

MA5-12135 was used in immunohistochemistry to report on two cases of sinonasal teratocarcinosarcoma

Fri Feb 01 00:00:00 UTC 2013

Chemical castration and anti-androgens induce differential gene expression in prostate cancer.

The Journal of pathology

Lehmusvaara S,Erkkilä T,Urbanucci A,Waltering K,Seppälä J,Larjo A,Tuominen VJ,Isola J,Kujala P,Lähdesmäki H,Kaipia A,Tammela TLj,Visakorpi T

MA5-12135 was used in immunohistochemistry to study the differential effects of chemical castration and anti-androgens on gene expression in prostate cancer

Sun Jul 01 00:00:00 UTC 2012

Identification of distinct gene expression profiles between esophageal squamous cell carcinoma and adjacent normal epithelial tissues.

The Tohoku journal of experimental medicine

Tao Y,Chai D,Ma L,Zhang T,Feng Z,Cheng Z,Wu S,Qin Y,Lai M

MA5-12135 was used in immunohistochemistry to use cDNA arrays to study the distinct gene expression profiles of esophageal squamous cell carcinoma cells and the surrounding normal tissue

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A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.

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Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ

MA5-12135 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence

Fri Jun 01 00:00:00 UTC 2012

Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.

Journal of neuro-oncology

Chen HK,Wu YT,Lin YJ,Lin JW

MA5-12135 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior

Fri Jul 01 00:00:00 UTC 2011

Routine dual-color immunostaining with a 3-antibody cocktail improves the detection of small cancers in prostate needle biopsies.

Human pathology

Tolonen TT,Kujala PM,Laurila M,Tirkkonen M,Ilvesaro J,Tuominen VJ,Tammela TL,Isola J

MA5-12135 was used in immunohistochemistry to evaluate a dual-color immunostaining protocol to detect small cancers in prostate needle biopsies

Tue Nov 01 00:00:00 UTC 2011

The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.

Indian journal of pathology & microbiology

Lin JW,Wu YT,Chang IW

MA5-12135 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma

Thu Apr 12 00:00:00 UTC 2012

Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.

Journal of neuro-oncology

Lin JW,Ho JT,Lin YJ,Wu YT

MA5-12135 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma

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Differences in the structural features of atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma.

Indian journal of urology : IJU : journal of the Urological Society of India

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MA5-12135 was used in immunohistochemistry to investigate the diagnostic histological differences between atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma

Tue Apr 01 00:00:00 UTC 2008

Chronic inflammation in benign prostate hyperplasia is associated with focal upregulation of cyclooxygenase-2, Bcl-2, and cell proliferation in the glandular epithelium.

The Prostate

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MA5-12135 was used in immunohistochemistry to study the upregulation of inflammatory pathways in benign prostatic hyperplasia and their possible role in prostate cancer pathogenesis

Wed Sep 15 00:00:00 UTC 2004

生物信息学

蛋白别名: 58 kDa cytokeratin; 67 kDa cytokeratin; CK-1; CK-10; CK-14; CK-5; cytokeratin 1; cytokeratin 10; cytokeratin 14; Cytokeratin-1; Cytokeratin-10; Cytokeratin-14; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Hair alpha protein; K1; K10; K14; K5; keratin 1, type II; keratin 10, type I; keratin 14, type I; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 5; Keratin-1; Keratin-10; Keratin-14; Keratin-5; Type-II keratin Kb1; Type-II keratin Kb5

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基因别名: BCIE; BIE; CK1; CK10; CK14; CK5; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; EPPK; K1; K10; K14; K5; KPP; KRT1; KRT10; KRT14; KRT1A; KRT5; KRT5A; KRTA; NEPPK; NFJ