Invitrogen

Cytokeratin Pan Type II Monoclonal Antibody (AE3), eBioscience™

Cytokeratin Pan Type II Antibody in Western Blot (WB)

FIGURE: 1 / 1

Cytokeratin Pan Type II Antibody (14-9000-82) in WB

MCF7 cell lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Basic Cytokeratin Purified.Bands were visualized using Anti-Mouse IgG HRP.

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Product Details

14-9000-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

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Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1834479

Product Specific Information

Description: The monoclonal antibody AE3 recognizes many members of the basic (Type II) cytokeratin family. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm.

The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins (also known as cytokeratin 1, 2, 3, 4, 5, 6, 7 and 8) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken.

Applications Reported: This AE3 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This AE3 antibody has been by tested western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Flow cytometry dataset for cells collected from touched surfaces.

F1000Research

Kwon YJ,Stanciu CE,Philpott MK,Ehrhardt CJ

14900082 was used in flow cytometry to examine epithelial cell populations from touch samples

Tue Dec 15 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; alpha keratin; CK-1; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-72; CK-8; cytokeratin 1; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; Cytokeratin otokeratin; Cytokeratin-1; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-72; Cytokeratin-8; Cytoskeletal 57 kDa keratin; ear specific cytokeratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; epithelial keratin 1; epithelial keratin 2e; Epithelial keratin-1; Epithelial keratin-2e; Hair alpha protein; K1; K2e; K2P; K3; K3 keratin; K4; K5; K6A; K7; K72; K76; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 2 epidermis; keratin 2, type II; keratin 2A (epidermal ichthyosis bullosa of Siemens); keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6, inner root sheath, 2; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 72, pseudogene; keratin 72, type II; keratin 76, type II; keratin 8, type II; keratin 86; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 35; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; keratin K3; keratin K6h; keratin protein K6irs; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 72; Keratin, type II cytoskeletal 8; Keratin, type II cytoskeletal cochleal; Keratin-1; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6-alpha; Keratin-6A; keratin-6C; Keratin-7; Keratin-72; Keratin-76; Keratin-8; krt2; krt5; krt7; krt76; masticatory epithelia keratin 2p; MGC116967; MGC116968; mK6-alpha; otokeratin; pan keratin; Sarcolectin; tuftelin interacting protein 8; type II alpha-keratin IIA; type II alpha-keratin IIC; type II cytoskeletal cochleal; Type II inner root sheath-specific keratin-K6irs2; type II mesothelial keratin K7; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II keratin Kb35; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9

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Gene Aliases: 2310001L23Rik; 2310016L08Rik; 3300001P10Rik; AA960620; AI507495; AL022697; AU019895; AW108092; AW146334; BB005427; CARD2; CK-2e; CK-3; CK-4; CK-6C; CK-6E; CK-8; CK1; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK4; CYK8; D15Wsu77e; DDD; DDD1; EBS2; EGK_03684; EGK_03685; EHK; EHK1; EndoA; EPPK; HUMCYT2A; K1; K2C7; K2C8; K2E; K3; K4; K5; K6A; K6C; K6D; K6irs; K6IRS2; K7; K8; KB35; KER1; Ker2; keratin; KO; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; KRT1A; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-35; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; Krt2-8; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6; KRT6A; KRT6C; KRT6D; KRT6IRS2; KRT7; KRT72; Krt72-ps; KRT76; KRT8; Krt86; KRTA; KRTE; mK6[a]; MK6a; NEPPK; PC3; SCL; Tfip8; WSN1

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Entrez Gene ID: (Dog) 444857, (Rhesus monkey) 699320, (Rabbit) 100353415, (Human) 3848, (Rabbit) 100353162, (Rhesus monkey) 699567, (Human) 3849, (Dog) 444850, (Human) 3850, (Dog) 100683401, (Rabbit) 100353668, (Rhesus monkey) 100425096, (Human) 3851, (Dog) 100682832, (Rhesus monkey) 700060, (Rabbit) 100348388, (Human) 3852, (Rhesus monkey) 100424400, (Chicken) 407779, (Rabbit) 100352658, (Dog) 100687760, (Dog) 486523, (Chicken) 408041, (Human) 3853, (Dog) 477602, (Human) 3855, (Chicken) 395772, (Rabbit) 100345579, (Rhesus monkey) 697425, (Dog) 486520, (Human) 140807, (Rhesus monkey) 718960, (Rhesus monkey) 699194, (Human) 51350, (Dog) 486518, (Rabbit) 100348891, (Human) 3856, (Dog) 486513, (Rhesus monkey) 700449, (Chicken) 426896, (Mouse) 16678, (Mouse) 16681, (Mouse) 16682, (Mouse) 110308, (Mouse) 16687, (Mouse) 110310, (Mouse) 105866, (Mouse) 77055, (Mouse) 16691

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Function(s)

protein heterodimerization activity receptor activity structural molecule activity protein binding carbohydrate binding structural constituent of cytoskeleton scaffold protein binding molecular_function protein complex binding

Process(es)

epithelial cell differentiation intermediate filament cytoskeleton organization protein heterotetramerization viral process cytoskeleton organization negative regulation of epithelial cell proliferation epidermis development sebaceous gland development biological_process tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process hemidesmosome assembly complement activation, lectin pathway retina homeostasis response to oxidative stress fibrinolysis regulation of angiogenesis negative regulation of inflammatory response establishment of skin barrier embryo development keratinization keratinocyte activation keratinocyte proliferation keratinocyte migration cell morphogenesis involved in differentiation multicellular organism development morphogenesis of an epithelium Wnt signaling pathway wound healing intermediate filament organization positive regulation of epidermis development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin Pan Type II Monoclonal Antibody (AE3), eBioscience™

Cytokeratin Pan Type II Antibody (14-9000-82) in WB

Product Details

14-9000-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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