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Invitrogen
DARC Recombinant Rabbit Monoclonal Antibody (10H52L38)
FIGURE: 1 / 3
DARC Antibody (703703) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous DARC using Anti-DARC Recombinant Rabbit Monoclonal Antibody (Product # 703703, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # 703404, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DARC protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962... View More
Product Details
703703
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H52L38
Immunogen
Protein corresponding to human DARC [aa1-aa63]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809247
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ACKR1 or DARC is an atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR1 is also known as an interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. It has a promiscuous chemokine-binding profile by interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. ACKR1 also acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and for the malaria parasites P.vivax and P.knowlesi.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Atypical chemokine receptor 1; CD234; CD234 antigen; CTA-134P22.3; Duffy Antigen; Duffy antigen chemokine receptor; Duffy antigen/chemokine receptor; Duffy blood group antigen; duffy blood group chemokine receptor; Duffy blood group, atypical chemokine receptor; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPDWBCQ1; GpFy; HGNC:4035; OTTHUMP00000060081; Plasmodium vivax receptor
Gene Aliases: ACKR1; CCBP1; CD234; DARC; Dfy; FY; GPD; GpFy; WBCQ1
UniProt ID: (Human) Q16570
Entrez Gene ID: (Human) 2532
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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