Invitrogen

DBT Polyclonal Antibody

DBT Antibody in Immunocytochemistry (ICC/IF)

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DBT Antibody (PA5-29727) in ICC/IF

Immunofluorescence analysis of DBT was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with DBT Polyclonal Antibody (Product # PA5-29727) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More

DBT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DBT Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. LNCaP cells were transfected with DBT siRNA and reduction of signal was observed in Western Blot using DBT Polyclonal Antibody (Product # PA5-29727). {KD}

产品信息

PA5-29727

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 11 and 247 of Human DBT

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.3 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547201

产品详细信息

Recommended positive controls: H1299, HepG2, mouse liver, rat liver.

Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (82%), Xenopus laevis (88%), Chicken (84%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cell-autonomous effect of cardiomyocyte branched-chain amino acid catabolism in heart failure in mice.

Acta pharmacologica Sinica

Yu JY,Cao N,Rau CD,Lee RP,Yang J,Flach RJR,Petersen L,Zhu C,Pak YL,Miller RA,Liu Y,Wang Y,Li Z,Sun H,Gao C

PA5-29727 was used in Western Blotting to establish the cardiomyocyte cell autonomous role of BCAA catabolism in cardiac physiology.

Sat Jul 01 00:00:00 UTC 2023

Genomic and biochemical analysis of repeatedly observed variants in DBT in individuals with maple syrup urine disease of Central American ancestry.

American journal of medical genetics. Part A

Billington CJ,Chapman KA,Leon E,Meltzer BW,Berger SI,Olson M,Figler RA,Hoang SA,Wanxing C,Wamhoff BR,Collado MS,Cusmano-Ozog K

PA5-29727 was used in Western Blotting to analyze liver cells and hepatocytes in a case series involving eleven individuals with Maple syrup urine disease (MSUD) caused by specific genetic variants, revealing decreased protein product levels and amino acid accumulation, all of whom were from Honduran or Salvadoran immigrant families, possibly indicating a founder effect.

Thu Sep 01 00:00:00 UTC 2022

Mitochondrial lipoylation integrates age-associated decline in brown fat thermogenesis.

Nature metabolism

Tajima K,Ikeda K,Chang HY,Chang CH,Yoneshiro T,Oguri Y,Jun H,Wu J,Ishihama Y,Kajimura S

PA5-29727 was used in Western Blotting to identify mitochondria lipoylation as a previously unappreciated molecular hallmark of aged brown adipose tissue (BAT) in mice.

Sun Sep 01 00:00:00 UTC 2019

生物信息学

蛋白别名: 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; BCKAD E2; BCKAD E2 subunit; BCKAD-E2; BCKADE2; BCOADC-E2; Branched chain 2-oxo-acid dehydrogenase complex component E2; branched chain acyltransferase, E2 component; Branched-chain alpha-keto acid dehydrogenase complex component E2; branched-chain alpha-ketoacid dehydrogenase, E2 subunit; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; dihydrolipoyl transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; E2 component of branched chain alpha-keto acid dehydrogenase complex; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex; MGC9061; mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b); part of the BCKAD complex

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基因别名: BCATE2; BCKAD-E2; BCKADE2; BCKDHE2; BCOADC-E2; D3Wsu60e; DBT; E2; E2B

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UniProt ID: (Human) P11182, (Mouse) P53395

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Entrez Gene ID: (Human) 1629, (Mouse) 13171, (Rat) 29611

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功能

ubiquitin protein ligase binding dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity transferase activity transferase activity, transferring acyl groups molecular_function acetyltransferase

参与通路

branched-chain amino acid catabolic process glyoxylate metabolic process metabolic process biological_process