Invitrogen

DDAH1 Polyclonal Antibody

DDAH1 Antibody in Immunocytochemistry (ICC/IF)

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DDAH1 Antibody (PA5-35306) in ICC/IF

Immunocytochemistry analysis of DDAH1 in HepG2 cells. Samples were incubated in DDAH1 polyclonal antibody (Product # PA5-35306) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

DDAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-35306

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:10-1:50

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2552616

产品详细信息

This antibody is predicted to react with bovine and rat based on sequence homology.

靶标信息

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

DDAH1 regulates apoptosis and angiogenesis in human fetal pulmonary microvascular endothelial cells.

Physiological reports

Trittmann JK,Almazroue H,Jin Y,Nelin LD

PA5-35306 was used in Western Blotting to test if DDAH1 expression is essential for maintaining nitric oxide production in human fetal pulmonary microvascular endothelial cells.

Mon Jul 01 00:00:00 UTC 2019

DDAH1 regulates apoptosis and angiogenesis in human fetal pulmonary microvascular endothelial cells.

Physiological reports

Trittmann JK,Almazroue H,Jin Y,Nelin LD

PA5-35306 was used in Western Blotting to test if DDAH1 expression is essential for maintaining nitric oxide production in human fetal pulmonary microvascular endothelial cells.

Mon Jul 01 00:00:00 UTC 2019

生物信息学

蛋白别名: DDAH-1; DDAHI; Dimethylargininase-1; epididymis secretory protein Li 16; FLJ21264; FLJ25539; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; NG, NG-dimethylarginine dimethylaminohydrolase; RP4-621F18.1

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基因别名: 2410006N07Rik; 2510015N06Rik; AI987801; AW050362; DDAH; DDAH1; HEL-S-16

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UniProt ID: (Human) O94760, (Mouse) Q9CWS0

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Entrez Gene ID: (Human) 23576, (Mouse) 69219

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功能

catalytic activity dimethylargininase activity amino acid binding metal ion binding zinc ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines hydrolase

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