Invitrogen

DDB1 Monoclonal Antibody (2B12D1)

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DDB1 Antibody (37-6200) in WB

Western blot analysis was performed on whole cell extracts of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), NIH/3T3 (Lane 5), Hep G2 (Lane 6). The blots were probed with Anti- DDB1 Mouse Monoclonal (Product # 37-6200, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 130 kDa band corresponding to DDB1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using ... View More

DDB1 Antibody in Immunoprecipitation (IP)

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DDB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DDB1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using DDB1 Mouse Monoclonal Antibody (Product # 37-6200). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

产品信息

37-6200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2B12D1

抗原

Recombinant human DDB1 protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533331

靶标信息

The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins.

Nature communications

Kumar N,Theil AF,Roginskaya V,Ali Y,Calderon M,Watkins SC,Barnes RP,Opresko PL,Pines A,Lans H,Vermeulen W,Van Houten B

37-6200 was used in Western Blot to propose that DDB2-mediated chromatin decompaction facilitates the recruitment of downstream BER proteins to 8-oxoG lesions.

Mon Feb 21 00:00:00 UTC 2022

HIV-1 Vpr counteracts HLTF-mediated restriction of HIV-1 infection in T cells.

Proceedings of the National Academy of Sciences of the United States of America

Yan J,Shun MC,Zhang Y,Hao C,Skowronski J

37-6200 was used in Western Blotting to study the role of Vpr targets in HIV-1 replication.

Tue May 07 00:00:00 UTC 2019

Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase.

Science advances

Hou L,Zhao J,Gao S,Ji T,Song T,Li Y,Wang J,Geng C,Long M,Chen J,Lin H,Cai X,Cang Y

Published figure using DDB1 monoclonal antibody (Product # 37-6200) in Western Blot

Fri Feb 01 00:00:00 UTC 2019

HIV-1 Vpr Reprograms CLR4^DCAF1 E3 Ubiquitin Ligase to Antagonize Exonuclease 1-Mediated Restriction of HIV-1 Infection.

mBio

Yan J,Shun MC,Hao C,Zhang Y,Qian J,Hrecka K,DeLucia M,Monnie C,Ahn J,Skowronski J

37-6200 was used in Western Blotting to determine the significance and role of human immunodeficiency virus type 1 accessory protein, Vpr, interactions in DNA repair.

Tue Oct 23 00:00:00 UTC 2018

CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory.

PLoS genetics

Song T,Liang S,Liu J,Zhang T,Yin Y,Geng C,Gao S,Feng Y,Xu H,Guo D,Roberts A,Gu Y,Cang Y

37-6200 was used in Immunoprecipitation to identify a competitive targeting of the BK channel to achieve control of its stability, and support changes in neuronal excitability as a common pathogenic mechanism underlying intellectual disability.

Mon Jan 01 00:00:00 UTC 2018

CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory.

PLoS genetics

Song T,Liang S,Liu J,Zhang T,Yin Y,Geng C,Gao S,Feng Y,Xu H,Guo D,Roberts A,Gu Y,Cang Y

Mon Jan 01 00:00:00 UTC 2018

SIRT7 deacetylates DDB1 and suppresses the activity of the CRL4 E3 ligase complexes.

The FEBS journal

Mo Y,Lin R,Liu P,Tan M,Xiong Y,Guan KL,Yuan HX

Published figure using DDB1 monoclonal antibody (Product # 37-6200) in Western Blot

Wed Nov 01 00:00:00 UTC 2017

Activation of c-Abl Kinase Potentiates the Anti-myeloma Drug Lenalidomide by Promoting DDA1 Protein Recruitment to the CRL4 Ubiquitin Ligase.

The Journal of biological chemistry

Gao S,Geng C,Song T,Lin X,Liu J,Cai Z,Cang Y

37-6200 was used in western blot to show that the Abl-DDB1-DDA1 axis reduces CRL4 substrate ubiquitination

Fri Mar 03 00:00:00 UTC 2017

HIV-1 and HIV-2 exhibit divergent interactions with HLTF and UNG2 DNA repair proteins.

Proceedings of the National Academy of Sciences of the United States of America

Hrecka K,Hao C,Shun MC,Kaur S,Swanson SK,Florens L,Washburn MP,Skowronski J

37-6200 was used in western blot to analyze the divergent interactions with UNG2 and HLTF DNA repair proteins by HIV-1 and HIV-2

Tue Jul 05 00:00:00 UTC 2016

CRL4A(CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis.

Nature communications

Liu J,Ye J,Zou X,Xu Z,Feng Y,Zou X,Chen Z,Li Y,Cang Y

37-6200 was used in western blot to investigate how ubiquitination of BK channels contributes to epilepsy.

Wed May 21 00:00:00 UTC 2014

Ubiquitin hydrolase UCH-L1 destabilizes mTOR complex 1 by antagonizing DDB1-CUL4-mediated ubiquitination of raptor.

Molecular and cellular biology

Hussain S,Feldman AL,Das C,Ziesmer SC,Ansell SM,Galardy PJ

Fri Mar 01 00:00:00 UTC 2013

Ubiquitin hydrolase UCH-L1 destabilizes mTOR complex 1 by antagonizing DDB1-CUL4-mediated ubiquitination of raptor.

Molecular and cellular biology

Hussain S,Feldman AL,Das C,Ziesmer SC,Ansell SM,Galardy PJ

Fri Mar 01 00:00:00 UTC 2013

Ubiquitin hydrolase UCH-L1 destabilizes mTOR complex 1 by antagonizing DDB1-CUL4-mediated ubiquitination of raptor.

Molecular and cellular biology

Hussain S,Feldman AL,Das C,Ziesmer SC,Ansell SM,Galardy PJ

Fri Mar 01 00:00:00 UTC 2013

Lentiviral Vpx accessory factor targets VprBP/DCAF1 substrate adaptor for cullin 4 E3 ubiquitin ligase to enable macrophage infection.

PLoS pathogens

Srivastava S,Swanson SK,Manel N,Florens L,Washburn MP,Skowronski J

37-6200 was used in western blot to suggest that lentiviral Vpx usurps VprBP-associated Cullin 4 ubiquitin ligase to allow for efficient reverse transcription

Fri May 09 00:00:00 UTC 2008

Lentiviral Vpr usurps Cul4-DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle.

Proceedings of the National Academy of Sciences of the United States of America

Hrecka K,Gierszewska M,Srivastava S,Kozaczkiewicz L,Swanson SK,Florens L,Washburn MP,Skowronski J

Published figure using DDB1 monoclonal antibody (Product # 37-6200) in Western Blot

Tue Jul 10 00:00:00 UTC 2007

生物信息学

蛋白别名: damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor

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基因别名: 127kDa; AA408517; DDB1; DDBA; p127-Ddb1; UV-DDB1; XAP1; XPCE; XPE; XPE-BF

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UniProt ID: (Human) Q16531, (Mouse) Q3U1J4

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Entrez Gene ID: (Human) 1642, (Mouse) 13194

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功能

DNA binding damaged DNA binding protein binding nucleic acid binding RNA processing factor

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