Invitrogen

DDT Polyclonal Antibody

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抗体检测数据 (13)

DDT Antibody in Immunocytochemistry (ICC/IF)

DDT Antibody in Immunocytochemistry (ICC/IF)

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DDT Antibody (PA5-95552) in ICC/IF

Immunocytochemistry analysis of DDT using anti-DDT antibody (Product # PA5-95552). DDT was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDT antibody (Product # PA5-95552)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize u... View More

DDT Antibody in Immunocytochemistry (ICC/IF)

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDT Antibody in Western Blot (WB)

DDT Antibody in Flow Cytometry (Flow)

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DDT Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DDT using anti-DDT antibody (Product # PA5-95552). DDT was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDT antibody (Product # PA5-95552)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human cholangiocarcinoma tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human ovary cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human lung cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human placenta tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded human tonsil tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded mouse spleen tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded rat spleen tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDT in paraffin-embedded rat brain tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDT Antibody in Western Blot (WB) — Western blot analysis of DDT in Lane 1: human HL-60 whole cell lysate, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with DDT polyclonal antibody (Product # PA5-95552) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

DDT Antibody in Flow Cytometry (Flow) — Flow Cytometry of DDT in U2OS cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with DDT Polyclonal Antibody (Product # PA5-95552) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-95552

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

免疫细胞化学 (ICC/IF)

2 µg/mL

-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence of human DDT (EFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2807354

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: D-dopachrome decarboxylase; D-dopachrome tautomerase; dopachrome isomerase; hCG_41098; Phenylpyruvate tautomerase II

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基因别名: C78655; DDCT; DDT

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UniProt ID: (Human) P30046, (Rat) P80254, (Mouse) O35215

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Entrez Gene ID: (Human) 1652, (Rat) 29318, (Mouse) 13202

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dopachrome isomerase activity D-dopachrome decarboxylase activity lyase activity decarboxylase

inflammatory response melanin biosynthetic process