Invitrogen

DDX1 Recombinant Rabbit Monoclonal Antibody (13H24L5)

图: 1 / 5

DDX1 Antibody (703141) in WB

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2), SK-N-AS (Lane 3), HL-60 (Lane 4), U-2 OS (Lane 5), Neuro-2a (Lane 6), A431 (Lane 7) and tissue extract of Mouse Brain (Lane 8). The blot was probed with Anti-DDX1 Recombinant Rabbit Monoclonal Antibody (Product # 703141, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~82 kDa band corres... View More

DDX1 Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

DDX1 Antibody — Antibody specificity was demonstrated by knockdown (KD) of target protein. Loss of signal was observed for target protein in DDX KD cell lysate using DDX1 Recombinant Rabbit Monoclonal Antibody (13H24L5) (Product # 703141). Ponceau stained transfer is shown below. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout or knockdown validation. {KD}

产品信息

703141

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

RNA免疫共沉淀 (RIP)

2 µg/10^6 cells

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

13H24L5

抗原

Protein corresponding to human DDX1 [aa72-aa283]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784565

产品详细信息

This antibody is predicted to react with Monkey, Cat, Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1

查看更多收起

基因别名: AA409185; DBP-RB; DDX1; UKVH5d

查看更多收起

UniProt ID: (Human) Q92499, (Mouse) Q91VR5

查看更多收起

Entrez Gene ID: (Human) 1653, (Mouse) 104721

查看更多收起

功能