Invitrogen

DDX5 Monoclonal Antibody (Ab204), Alexa Fluor™ 647, eBioscience™

图: 1 / 2

DDX5 Antibody (51-6005-42) in Flow

Normal human peripheral blood cells were stimulated for 1 day with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) and stained with eBioscience Fixable Viability Dye eFluor 450 (Product #65-0863-14). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD3 Monoclonal Antibody, FITC (Product # 11-0038-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-81) (blue histogram) or DDX5 Monoclona... View More

Product Image Gallery

DDX5 Antibody — Normal human peripheral blood cells were unstimulated (orange histogram) or stimulated for 1 day with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with Mouse IgG1 kappa Isotype Control, (blue histogram) or DDX5 Monoclonal Antibody (purple histogram). Cells in the lymphocyte gate were used for analysis. {RE}

产品信息

51-6005-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µl (0.25 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

Ab204

抗原

Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells

查看免疫原

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2802308

产品详细信息

Description: This Ab204 monoclonal antibody detects human DDX5 protein also known as DEAD box protein 5 and RNA helicase p68. This antibody has been reported to cross-react with mouse and rat orthologs of DDX5 protein.

Applications Reported: This Ab204 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Ab204 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Applications Reported: This Ab204 antibody has been reported for use in immunocytochemical staining.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

靶标信息

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; MGC118083; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4

查看更多收起

基因别名: DDX5; G17P1; HELR; HLR1; HUMP68; p68

查看更多收起

UniProt ID: (Human) P17844

查看更多收起

Entrez Gene ID: (Human) 1655

查看更多收起

功能