Invitrogen

DDX6 Polyclonal Antibody

DDX6 Antibody in Immunocytochemistry (ICC/IF)

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DDX6 Antibody (PA5-18478) in ICC/IF

Immunofluorescence analysis of DDX6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DDX6 Polyclonal Antibody (Product # PA5-18478) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32814) at 1... View More

DDX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DDX6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DDX6 siRNA and decrease in signal intensity was observed in western blot application using DDX6 Polyclonal Antibody (Product # PA5-18478). {KD}

产品信息

PA5-18478

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.3-1 µg/mL

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (AEYHSEPVEDEKP) corresponding to the C-terminus amino acids of DDX6 (aa 471-483).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10985968

产品详细信息

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

靶标信息

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD-box protein 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); Oncogene RCK; Probable ATP-dependent RNA helicase DDX6

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基因别名: DDX6; HLR2; P54; RCK

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UniProt ID: (Human) P26196

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Entrez Gene ID: (Human) 1656

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功能

RNA helicase activity ATP-dependent RNA helicase activity helicase activity protein binding ATP binding protein domain specific binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion

参与通路

regulation of translation RNA secondary structure unwinding viral RNA genome packaging stem cell population maintenance cytoplasmic mRNA processing body assembly exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay negative regulation of neuron differentiation cell-cell adhesion