Invitrogen

DDX6 Polyclonal Antibody

DDX6 Antibody in Immunocytochemistry (ICC/IF)

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DDX6 Antibody (PA5-55012) in ICC/IF

Immunofluorescence analysis of DDX6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DDX6 Polyclonal Antibody (Product # PA5-55012) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at... View More

DDX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDX6 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-55012

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human DDX6. Recombinant protein control fragment (Product #RP-92557).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2640486

产品详细信息

Immunogen sequence: ENPVIMGLSS QNGQLRGPVK PTGGPGGGGT QTQQQMNQLK NTNTINNGTQ QQAQSMTTTI KPGDDWKKTL KLPPKDLRIK TSDVTSTKGN EFEDYCLKRE LLMGIFEMGW EKPSPIQ

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 93%.

靶标信息

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Peroxynitrite in the tumor microenvironment changes the profile of antigens allowing escape from cancer immunotherapy.

Cancer cell

Tcyganov EN,Sanseviero E,Marvel D,Beer T,Tang HY,Hembach P,Speicher DW,Zhang Q,Donthireddy LR,Mostafa A,Tsyganova S,Pisarev V,Laufer T,Ignatov D,Ferrone S,Meyer C,Maby-El Hajjami H,Speiser DE,Altiok S,Antonia S,Xu X,Xu W,Zheng C,Schuchter LM,Amaravadi RK,Mitchell TC,Karakousis GC,Yuan Z,Montaner LJ,Celis E,Gabrilovich DI

PA5-55012 was used in Western Blot to suggest that intratumoral PNT activity should be considered for the design of neoantigen-based therapy and also may be an important immunotherapeutic target.

Mon Oct 10 00:00:00 UTC 2022

生物信息学

蛋白别名: ATP-dependent RNA helicase p54; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 6; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD-box protein 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); Oncogene RCK; Oncogene RCK homolog; Probable ATP-dependent RNA helicase DDX6

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基因别名: 1110001P04Rik; DDX6; E230023J21Rik; HLR2; mRCK/P54; P54; RCK; RGD1564560

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UniProt ID: (Human) P26196, (Mouse) P54823

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Entrez Gene ID: (Human) 1656, (Mouse) 13209, (Rat) 500988

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