Invitrogen

DEGS2 Polyclonal Antibody

DEGS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DEGS2 Antibody (PA5-24082) in IHC (P)

Immunohistochemistry analysis of DEGS2 in formalin fixed and paraffin embedded human kidney tissue. Samples were incubated with DEGS2 polyclonal antibody (Product # PA5-24082) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

DEGS2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

DEGS2 Antibody — Depletion of DES2 recapitulates most of defects observed upon depletion of CD98hc. a Depletion of DES2 by siRNA impairs RhoA activation by mechanical force application on integrins. Right panel, quantification of RhoA activation, means are plotted with s.e.m. as error bars from n = 2 experiments, *P < 0.05 in a two-way ANOVA. b Depletion of DES2 by shRNA impairs SFK phosphorylation at Y416. One experiment representative of n = 2. c Depletion of DES2 impairs mechanically induced GEF-H1 activation. One experiment representative of n = 2. d Depletion of DES2 triggers GEF-H1 sequestration in cell membrane. s supernatant, p membrane pellet. One experiment representative of n = 2. e DES2 depletion partially inhibits mechanically coupled YAP/Taz activation as measured by RT-qPCR of ANKRD1 transcript. Means are plotted with s.e.m. as error bars from n = 6 with *P < 0.05 and ****P < 0.0001 in a two-way ANOVA. f Depletion of DES2 impairs generation of traction forces. Traction force microscopy was performed on subconfluent cells. Scale bar is 50 µm {KD}

Product Details

PA5-24082

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 261-290 amino acids from the C-terminal region of human DEGS2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2541582

Product Specific Information

This antibody is predicted to react with bovine and mouse based on sequence homology.

Target Information

DEGS2 encodes a bifunctional enzyme that is involvedin the biosynthesis of phytosphingolipids in human skin and inother phytosphingolipid-containing tissues. This enzyme can act asa sphingolipid delta(4)-desaturase, and also as a sphingolipidC4-hydroxylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Urinary Bladder is Rich in Glycosphingolipids Composed of Phytoceramides.

Journal of lipid research

Watanabe T,Suzuki A,Ohira S,Go S,Ishizuka Y,Moriya T,Miyaji Y,Nakatsuka T,Hirata K,Nagai A,Matsuda J

PA5-24082 was used in Immunohistochemistry (Paraffin), Western Blot to report the structural variation in GSLs in the urinary bladders of mice and humans.

Thu Dec 01 00:00:00 UTC 2022

The Urinary Bladder is Rich in Glycosphingolipids Composed of Phytoceramides.

Journal of lipid research

Watanabe T,Suzuki A,Ohira S,Go S,Ishizuka Y,Moriya T,Miyaji Y,Nakatsuka T,Hirata K,Nagai A,Matsuda J

PA5-24082 was used in Immunohistochemistry (Paraffin), Western Blot to report the structural variation in GSLs in the urinary bladders of mice and humans.

Thu Dec 01 00:00:00 UTC 2022

Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway.

Nature communications

Boulter E,Estrach S,Tissot FS,Hennrich ML,Tosello L,Cailleteau L,de la Ballina LR,Pisano S,Gavin AC,Féral CC

Published figure using DEGS2 polyclonal antibody (Product # PA5-24082) in Western Blot

Mon Nov 19 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Degenerative spermatocyte homolog 2; degenerative spermatocyte homolog 2, lipid desaturase; DEGS2; delta(4)-desaturase, sphingolipid 2; dihydroceramide desaturase 2; Sphingolipid 4-desaturase; sphingolipid C4-hydroxylase/delta 4-desaturase; Sphingolipid C4-monooxygenase; sphingolipid delta 4 desaturase/C-4 hydroxylase; sphingolipid delta(4)-desaturase 2; sphingolipid delta(4)-desaturase/C4-hydroxylase DES2; Sphingolipid delta(4)-desaturase/C4-monooxygenase DES2

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Gene Aliases: C14orf66; DEGS2; DES2; FADS8

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UniProt ID: (Human) Q6QHC5

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Entrez Gene ID: (Human) 123099

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Function(s)

sphingosine hydroxylase activity sphingolipid delta-4 desaturase activity

Process(es)

sphinganine metabolic process sphingolipid biosynthetic process ceramide biosynthetic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DEGS2 Polyclonal Antibody

DEGS2 Antibody (PA5-24082) in IHC (P)

Product Details

PA5-24082

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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