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DGCR8 Antibody (PA5-40122) in ICC/IF
Immunofluorescence analysis of DGCR8 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DGCR8 Polyclonal Antibody (Product # PA5-40122) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of... View More
产品信息
PA5-40122
应用
建议稀释比
已发表文章
产品规格
种属反应
Amphibian,
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8.
偶联物
形式
Liquid
浓度
1.15 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2606318
产品详细信息
Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.
Predicted reactivity: Mouse (82%), Rat (80%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Microprocessor complex subunit DGCR8
基因别名: C22orf12; DGCR8; DGCRK6; Gy1; LP4941; pasha
UniProt ID: (Human) Q8WYQ5
Entrez Gene ID: (Human) 54487
