Invitrogen

DHCR7 Polyclonal Antibody

DHCR7 Antibody in Immunocytochemistry (ICC/IF)

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DHCR7 Antibody (PA5-48204) in ICC/IF

Immunocytochemistry analysis of DHCR7 in Hela (Human Cervical epithelial adenocarcinoma cell line) cells. Samples were incubated with DHCR7 polyclonal antibody (Product # PA5-48204) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter st... View More

DHCR7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-48204

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:10-1:50

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2633662

靶标信息

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Chronic Aripiprazole and Trazodone Polypharmacy Effects on Systemic and Brain Cholesterol Biosynthesis.

Biomolecules

Korade Z,Anderson A,Balog M,Tallman KA,Porter NA,Mirnics K

Published figure using DHCR7 polyclonal antibody (Product # PA5-48204) in Western Blot

Mon Aug 28 00:00:00 UTC 2023

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

The Journal of cell biology

Findakly S,Daggubati V,Garcia G,LaStella SA,Choudhury A,Tran C,Li A,Tong P,Garcia JQ,Puri N,Reiter JF,Xu L,Raleigh DR

PA5-48204 was used in Immunocytochemistry to identify an enzyme involved in lipid biosynthesis which acts as an activator of the Hedgehog pathway.

Mon Jan 04 00:00:00 UTC 2021

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

The Journal of cell biology

Findakly S,Daggubati V,Garcia G,LaStella SA,Choudhury A,Tran C,Li A,Tong P,Garcia JQ,Puri N,Reiter JF,Xu L,Raleigh DR

PA5-48204 was used in Immunocytochemistry to identify an enzyme involved in lipid biosynthesis which acts as an activator of the Hedgehog pathway.

Mon Jan 04 00:00:00 UTC 2021

Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression.

Experimental cell research

Kohlhaas J,Jäger MA,Lust L,De La Torre C,Hecker M,Korff T

PA5-48204 was used in Simple Western - Size to indicate that ECs have the capacity to instruct VSMCs to shut down the expression of DHCR24 thereby limiting their cholesterol biosynthesis, which may support their functional steady state.

Mon Feb 15 00:00:00 UTC 2021

生物信息学

蛋白别名: 7-dehydrocholesterol reductase; 7-DHC reductase; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; putative sterol reductase SR-2; Sterol Delta(7)-reductase; sterol delta-7-reductase; Sterol reductase SR-2

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基因别名: AA409147; D7SR; DHCR7; SLOS

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UniProt ID: (Human) Q9UBM7, (Mouse) O88455

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Entrez Gene ID: (Human) 1717, (Mouse) 13360

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功能

7-dehydrocholesterol reductase activity NADP binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor reductase

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