Invitrogen

DIAPH1 Polyclonal Antibody

DIAPH1 Antibody in Immunocytochemistry (ICC/IF)

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DIAPH1 Antibody (PA5-21409) in ICC/IF

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Product # PA5-21409) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

DIAPH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-21409

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 237 of DIAPH1 (Uniprot ID#O60610)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.69 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153295

产品详细信息

Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (95%), Rat (94%), Pig (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.

eLife

Bolado-Carrancio A,Rukhlenko OS,Nikonova E,Tsyganov MA,Wheeler A,Garcia-Munoz A,Kolch W,von Kriegsheim A,Kholodenko BN

PA5-21409 was used in Western Blotting to elaborate the minimal autonomous biochemical machinery necessary and sufficient for dynamic trailing edge coordination and cell movement.

Fri Jul 24 00:00:00 UTC 2020

Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.

eLife

Bolado-Carrancio A,Rukhlenko OS,Nikonova E,Tsyganov MA,Wheeler A,Garcia-Munoz A,Kolch W,von Kriegsheim A,Kholodenko BN

PA5-21409 was used in Western Blotting to elaborate the minimal autonomous biochemical machinery necessary and sufficient for dynamic trailing edge coordination and cell movement.

Fri Jul 24 00:00:00 UTC 2020

Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish.

Nature communications

Castanon I,Hannich JT,Abrami L,Huber F,Dubois M,Müller M,van der Goot FG,Gonzalez-Gaitan M

PA5-21409 was used in Western Blotting to show how Wnt signaling mediates sphingolipid-dependent oriented division and how sphingolipids determine Anthrax receptor palmitoylation.